Multiparametric evaluation of apoptosis: effects of standard cytotoxic agents and the cyanoguanidine CHS 828.
AuthorsLövborg H, Nygren P, Larsson R
JournalMol Cancer Ther
PubMed ID15141009
'A multiparametric high-content screening assay for measurement of apoptosis was developed. HeLa cells and lymphoma U-937 cells were exposed to cytotoxic drugs in flat-bottomed optical microtiter plates. After incubation, the DNA-binding dye Hoechst 33342, fluorescein-tagged probes that covalently bind active caspases and chloromethyl-X-rosamine to detect mitochondrial membrane potential (MMP) were ... More
MiR-351 transiently increases during muscle regeneration and promotes progenitor cell proliferation and survival upon differentiation.
AuthorsChen Y, Melton DW, Gelfond JA, McManus LM, Shireman PK,
JournalPhysiol Genomics
PubMed ID22968638
MicroRNAs (miRNAs) regulate many biological processes including muscle development. However, little is known regarding miRNA regulation of muscle regeneration. Murine tibialis anterior muscle was evaluated after cardiotoxin-induced injury and used for global miRNA expression analysis. From day 1 through day 21 following injury, 298 miRNAs were significantly changed at least ... More
Streptococcus pneumoniae evades human dendritic cell surveillance by pneumolysin expression.
AuthorsLittmann M, Albiger B, Frentzen A, Normark S, Henriques-Normark B, Plant L,
JournalEMBO Mol Med
PubMed ID20049723
Dendritic cells (DCs) protect the respiratory epithelium via induction of innate immune responses and priming of naïve T cells during the initiation of adaptive immunity. Streptococcus pneumoniae, a commonly carried asymptomatic member of the human nasopharyngeal microflora, can cause invasive and inflammatory diseases and the cholesterol-dependent cytotoxin pneumolysin is a ... More
Marked inhibition of growth and invasive parameters of head and neck squamous carcinoma FaDu by a nutrient mixture.
AuthorsRoomi MW, Roomi NW, Kalinovsky T, Rath M, Niedzwiecki A,
JournalIntegr Cancer Ther
PubMed ID19679626
Head and neck squamous cell carcinomas (HNSCCs) are known for their aggressive growth and propensity to metastasize. The authors investigated the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HNSCC cell line FaDu in vivo and in vitro. Athymic male ... More
The Effect of Axon Resealing on Retrograde Neuronal Death after Spinal Cord Injury in Lamprey.
Authors
JournalBrain Sci
PubMed ID29661988
RhoA activation in axotomy-induced neuronal death.
AuthorsZhang G, Hu J, Rodemer W, Li S, Selzer ME
JournalExp Neurol
PubMed ID29715475
'After spinal cord injury (SCI) in mammals, severed axons fail to regenerate, due to both extrinsic inhibitory factors, e.g., the chondroitin sulfate proteoglycans (CSPGs) and myelin-associated growth inhibitors (MAIs), and a developmental loss of intrinsic growth capacity. The latter is suggested by findings in lamprey that the 18 pairs of ... More
Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.
Authors
JournalEnzyme Res
PubMed ID22829997
The microenvironment of embryoid bodies modulated the commitment to neural lineage postcryopreservation.
Authors
JournalTissue Eng Part C Methods
PubMed ID25187378
GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys.
Authors
JournalCell Death Dis
PubMed ID29950557
Chondroitinase ABC Promotes Axon Regeneration and Reduces Retrograde Apoptosis Signaling in Lamprey.
Authors
JournalFront Cell Dev Biol
PubMed ID33842481
Astrocyte- and Neuron-Derived Extracellular Vesicles from Alzheimer's Disease Patients Effect Complement-Mediated Neurotoxicity.
Authors
JournalCells
PubMed ID32635578
Neural patterning of human induced pluripotent stem cells in 3-D cultures for studying biomolecule-directed differential cellular responses.
AuthorsYan Y, Bejoy J, Xia J, Guan J, Zhou Y, Li Y
JournalActa Biomater
PubMed ID27345135
Appropriate neural patterning of human induced pluripotent stem cells (hiPSCs) is critical to generate specific neural cells/tissues and even mini-brains that are physiologically relevant to model neurological diseases. However, the capacity of signaling factors that regulate 3-D neural tissue patterning in vitro and differential responses of the resulting neural populations ... More
A hemagglutinin isolated from Northeast China black beans induced mitochondrial dysfunction and apoptosis in colorectal cancer cells.
AuthorsDan X, Ng TB, Wong JH, Chan YS, Cheung RC, Chan WY
JournalBiochim Biophys Acta
PubMed ID27235832
Incidence of colorectal cancer is closely related with the lifestyle, especially the dietary habits of patients. Epidemiological researches have demonstrated a negative correlation between legume consumption and colorectal cancer incidence. Lectins/hemagglutinins are a type of carbohydrate binding proteins which are abundantly stored in legumes. Their eminent pH-stability allows them to ... More
Crosslinking of extracellular matrix scaffolds derived from pluripotent stem cell aggregates modulates neural differentiation.
AuthorsSart S, Yan Y, Li Y, Lochner E, Zeng C, Ma T, Li Y
JournalActa Biomater
PubMed ID26577988
At various developmental stages, pluripotent stem cells (PSCs) and their progeny secrete a large amount of extracellular matrices (ECMs) which could interact with regulatory growth factors to modulate stem cell lineage commitment. ECMs derived from PSC can be used as unique scaffolds that provide broad signaling capacities to mediate cellular ... More
Retrograde Activation of the Extrinsic Apoptotic Pathway in Spinal-Projecting Neurons after a Complete Spinal Cord Injury in Lampreys.
AuthorsBarreiro-Iglesias A, Sobrido-Cameán D, Shifman MI
JournalBiomed Res Int
PubMed ID29333445
Spinal cord injury (SCI) is a devastating condition that leads to permanent disability because injured axons do not regenerate across the trauma zone to reconnect to their targets. A prerequisite for axonal regeneration will be the prevention of retrograde degeneration that could lead to neuronal death. However, the specific molecular ... More
Data on the effect of a muscimol treatment in caspase activation in descending neurons of lampreys after a complete spinal cord injury.
AuthorsSobrido-Cameán D, Rodicio MC, Barreiro-Iglesias A
JournalData Brief
PubMed ID30510990
In this article, caspase activation in identifiable reticulospinal neurons of lampreys was inhibited after a complete spinal cord injury using a specific agonist of the GABAA receptor (muscimol). The data presented in this article are quantifications of fluorescent labelling of identifiable descending neurons of larval lampreys after a complete spinal ... More