Multiparametric evaluation of apoptosis: effects of standard cytotoxic agents and the cyanoguanidine CHS 828.
AuthorsLövborg H, Nygren P, Larsson R
JournalMol Cancer Ther
PubMed ID15141009
'A multiparametric high-content screening assay for measurement of apoptosis was developed. HeLa cells and lymphoma U-937 cells were exposed to cytotoxic drugs in flat-bottomed optical microtiter plates. After incubation, the DNA-binding dye Hoechst 33342, fluorescein-tagged probes that covalently bind active caspases and chloromethyl-X-rosamine to detect mitochondrial membrane potential (MMP) were ... More
Zinc-dependent multi-conductance channel activity in mitochondria isolated from ischemic brain.
AuthorsBonanni L, Chachar M, Jover-Mengual T, Li H, Jones A, Yokota H, Ofengeim D, Flannery RJ, Miyawaki T, Cho CH, Polster BM, Pypaert M, Hardwick JM, Sensi SL, Zukin RS, Jonas EA,
JournalJ Neurosci
PubMed ID16793892
'Transient global ischemia is a neuronal insult that induces delayed cell death. A hallmark event in the early post-ischemic period is enhanced permeability of mitochondrial membranes. The precise mechanisms by which mitochondrial function is disrupted are, as yet, unclear. Here we show that global ischemia promotes alterations in mitochondrial membrane ... More
Flow cytometry-based apoptosis detection.
AuthorsWlodkowic D, Skommer J, Darzynkiewicz Z,
JournalMethods Mol Biol
PubMed ID19609746
'An apoptosing cell demonstrates multitude of characteristic morphological and biochemical features, which vary depending on the stimuli and the cell type. The gross majority of classical apoptotic hallmarks can be rapidly examined by flow and image cytometry. Cytometry thus became a technology of choice in diverse studies of cellular demise. ... More
Molecular analysis and characterization of zebrafish keratocan (zKera) gene.
AuthorsYeh LK, Liu CY, Chien CL, Converse RL, Kao WW, Chen MS, Hu FR, Hsieh FJ, Wang IJ,
JournalJ Biol Chem
PubMed ID17965408
Corneal small leucine-rich proteoglycans play a pivotal role in maintaining corneal transparency and function. In this study, we isolated and characterized the zebrafish (Danio rerio) keratocan (zKera) gene. The human keratocan sequence was used to search zebrafish homologues. The zKera full-length genomic DNA and cDNA were generated via PCR of ... More
CXCR2 Mediates Distinct Neutrophil Behavior in Brain Metastatic Breast Tumor.
Authors
JournalCancers (Basel)
PubMed ID35158784
Larval Exposure to Chlorpyrifos Affects Nutritional Physiology and Induces Genotoxicity in Silkworm Philosamia ricini (Lepidoptera: Saturniidae).
Authors
JournalFront Physiol
PubMed ID27895594
TNT-Induced Phagocytosis: Tunneling Nanotubes Mediate the Transfer of Pro-Phagocytic Signals From Apoptotic to Viable Cells.
AuthorsBittins M, Wang X
JournalJ Cell Physiol
PubMed ID27591547
'The exposure of phosphatidylserine (PS) on the surface membrane of apoptotic cells triggers the recruitment of phagocytic receptors and subsequently results in uptake by phagocytes. Here we describe how apoptotic cells can use intercellular membrane nanotubes to transfer exposed PS to neighboring viable cells, and thus deposit an "eat-me" tag ... More
7-Ketocholesterol overcomes drug resistance in chronic myeloid leukemia cell lines beyond MDR1 mechanism.
'Chronic myeloid leukemia (CML) is a myeloproliferative disease with a characteristic BCR-ABL tyrosine kinase (TK) fusion protein. Despite the clinical efficacy accomplished by TKIs therapies, disease progression may affect patient response rate to these inhibitors due to a multitude of factors that could lead to development of a mechanism known ... More
Praziquantel induced oxidative stress and apoptosis-like cell death in Raillietina echinobothrida.
AuthorsGiri BR, Roy B
JournalActa Trop
PubMed ID27005397
'Praziquantel (PZQ) is an anthelmintic drug used against trematode and cestode parasites of humans and veterinary animals. Since praziquantel was introduced as a broadspectrum anthelmintic, numerous studies described its successful use against helminth parasites, but its exact mechanism of action is feebly understood. Therefore, the present study was carried out ... More
The Nuclear Matrix Protein Megator Regulates Stem Cell Asymmetric Division through the Mitotic Checkpoint Complex in Drosophila Testes.
Authors
JournalPLoS Genet
PubMed ID26714316
THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.
Authors
JournalJ Immunother Cancer
PubMed ID34341130
Suppression of PPN/MG61 attenuates Wnt/beta-catenin signaling pathway and induces apoptosis in human lung cancer.
Authors
JournalOncogene
PubMed ID18193088
Connexin43 is required for the maintenance of multipotency in skin-derived stem cells.
Authors
JournalStem Cells Dev
PubMed ID24694074
Cysticercus fasciolaris infection induced oxidative stress and apoptosis in rat liver: a strategy for host-parasite cross talk.
AuthorsGiri BR, Roy B
JournalParasitol Res
PubMed ID26987645
Parasitic helminths have developed various strategies to induce or inhibit apoptosis in the cells of their host, thereby modulating the host's immune response and aiding dissemination to the host. Cysticercus fasciolaris, the larval form of Taenia taeniaeformis, parasitized different intermediate hosts like rats, rabbits, etc. and is cosmopolitan in distribution. ... More
Retrograde Activation of the Extrinsic Apoptotic Pathway in Spinal-Projecting Neurons after a Complete Spinal Cord Injury in Lampreys.
AuthorsBarreiro-Iglesias A, Sobrido-Cameán D, Shifman MI
JournalBiomed Res Int
PubMed ID29333445
Spinal cord injury (SCI) is a devastating condition that leads to permanent disability because injured axons do not regenerate across the trauma zone to reconnect to their targets. A prerequisite for axonal regeneration will be the prevention of retrograde degeneration that could lead to neuronal death. However, the specific molecular ... More
Cypermethrin Formulation (Ustad-10 EC) Induces Genotoxicity via Apoptosis, Affects Nutritional Physiology, and Modulates Immune Response in Silkworm Philosamia ricini (Lepidoptera: Saturniidae).
AuthorsKalita MK, Haloi K, Devi D
JournalJ Econ Entomol
PubMed ID28369445
Cypermethrin is a pyrethroid insecticide with high insecticidal activity, low mammalian toxicity, and biodegradability. The present study aimed to determine the acute toxicity and evaluate the secondary toxic effects of a commercial formulation of cypermethrin on silkworm Philosamia ricini Hutt of Northeast India. The potential genotoxicity of cypermethrin on silkworm ... More
Characterization of KIF11 as a novel prognostic biomarker and therapeutic target for oral cancer.
AuthorsDaigo K, Takano A, Thang PM, Yoshitake Y, Shinohara M, Tohnai I, Murakami Y, Maegawa J, Daigo Y
JournalInt J Oncol
PubMed ID29115586
Oral cancer has a high mortality rate, and its incidence is increasing gradually worldwide. As the effectiveness of standard treatments is still limited, the development of new therapeutic strategies is eagerly awaited. Kinesin family member 11 (KIF11) is a motor protein required for establishing a bipolar spindle in cell division. The role ... More
Techniques for the Study of Apoptosis in Bone.
AuthorsMann V, Noble B
JournalMethods Mol Biol
PubMed ID30729482
Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in ... More