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Invitrogen™

Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy

Catalog number: I35106
Invitrogen™

Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy

Catalog number: I35106
Detect and evaluate caspase activation, membrane permeability, and cell cycle using Image-iT LIVE red and green poly and caspase-3 and-7 apoptosis detection kits for microscopy, including fluorescence microscopy and high content screening (HCS). The Image-iT LIVE red and green caspase detection kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity, a measure of cell apoptosis. Specifically, the Image-iT LIVE red and green caspase detection kits contain the substrates SR-VAD-FMK and SR-DEVD-FMK (for red fluorescence), or FAM-VAD-FMK and FAM-DEVD-FMK (green fluorescence), for poly caspases and caspase-3/7 detection, respectively. They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by microscopy and HCS.
 
Catalog Number
I35106
Unit Size
1 kit
Color
Green, Red, Blue
Enzyme
Caspase 3/7
Label or Dye
FAM-DEVD-FMK, propidium iodide (PI), Hoechst 33342
Price (USD)
288.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionImage-iT LIVE Green Caspase-3 and -7 Detection Kit
Product TypeCaspase 3/7 Reagent
Excitation/EmissionFAM-DEVD-FMK: 488/530
propidium iodide (PI): 535/617
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorGreen, Red, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeFAM-DEVD-FMK, propidium iodide (PI), Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymeCaspase 3/7
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Red, Green, Blue
Enzyme
Poly Caspases
Label or Dye
SR-VAD-FMK, SYTOX Green, Hoechst 33342
Price (USD)
424.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionImage-iT LIVE Red Poly Caspases Detection Kit
Product TypeCaspase Assay Kit
Excitation/EmissionSR-VAD-FMK: 550/595
SYTOX Green: 504/523
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorRed, Green, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeSR-VAD-FMK, SYTOX Green, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymePoly Caspases
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Red, Green, Blue
Enzyme
Caspase 3/7
Label or Dye
SR-DEVD-FMK, SYTOX Green, Hoechst 33342
Price (USD)
484.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionImage-iT LIVE Red Caspase-3 and -7 Detection Kit
FormatSlide
Product TypeCaspase 3/7 Reagent
Excitation/EmissionSR-DEVD-FMK: 550/595
SYTOX Green: 504/523
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorRed, Green, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeSR-DEVD-FMK, SYTOX Green, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymeCaspase 3/7
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Green, Red, Blue
Enzyme
Poly Caspases
Label or Dye
FAM-VAD-FMK, propidium iodide (PI), Hoechst 33342
Price (USD)
288.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionImage-iT LIVE Green Poly Caspases Detection Kit
FormatSlide
Product TypeCaspase Assay Kit
Excitation/EmissionFAM-VAD-FMK: 488/530
propidium iodide (PI): 535/617
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorGreen, Red, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeFAM-VAD-FMK, propidium iodide (PI), Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymePoly Caspases
Unit Size1 kit
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeColorEnzymeLabel or DyePrice (USD)AvailabilityQuantity
I35106Full specifications
1 kitGreen, Red, BlueCaspase 3/7FAM-DEVD-FMK, propidium iodide (PI), Hoechst 33342
288.00
Your Price:
***
DescriptionImage-iT LIVE Green Caspase-3 and -7 Detection Kit
Product TypeCaspase 3/7 Reagent
Excitation/EmissionFAM-DEVD-FMK: 488/530
propidium iodide (PI): 535/617
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorGreen, Red, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeFAM-DEVD-FMK, propidium iodide (PI), Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymeCaspase 3/7
Unit Size1 kit
I35101Full specifications
1 kitRed, Green, BluePoly CaspasesSR-VAD-FMK, SYTOX Green, Hoechst 33342
424.00
Your Price:
***
DescriptionImage-iT LIVE Red Poly Caspases Detection Kit
Product TypeCaspase Assay Kit
Excitation/EmissionSR-VAD-FMK: 550/595
SYTOX Green: 504/523
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorRed, Green, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeSR-VAD-FMK, SYTOX Green, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymePoly Caspases
Unit Size1 kit
I35102Full specifications
1 kitRed, Green, BlueCaspase 3/7SR-DEVD-FMK, SYTOX Green, Hoechst 33342
484.00
Your Price:
***
DescriptionImage-iT LIVE Red Caspase-3 and -7 Detection Kit
FormatSlide
Product TypeCaspase 3/7 Reagent
Excitation/EmissionSR-DEVD-FMK: 550/595
SYTOX Green: 504/523
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorRed, Green, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeSR-DEVD-FMK, SYTOX Green, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymeCaspase 3/7
Unit Size1 kit
I35104Full specifications
1 kitGreen, Red, BluePoly CaspasesFAM-VAD-FMK, propidium iodide (PI), Hoechst 33342
288.00
Your Price:
***
DescriptionImage-iT LIVE Green Poly Caspases Detection Kit
FormatSlide
Product TypeCaspase Assay Kit
Excitation/EmissionFAM-VAD-FMK: 488/530
propidium iodide (PI): 535/617
Hoechst 33342: 350/461
No. of Reactions25 tests (labeling volumes of 300µL)
ColorGreen, Red, Blue
Shipping ConditionRoom Temperature
Product LineImage-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeFAM-VAD-FMK, propidium iodide (PI), Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
EnzymePoly Caspases
Unit Size1 kit
Showing 4 of 4
The Image-iT LIVE red and green caspase detection kits for microscopy employ a novel approach to detecting active caspases: both assay kits take advantage of a fluorescent inhibitor of caspases (FLICA) methodology, which is an affinity label. This reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amino acid sequence. Different amino acid recognition sequences are used to detect different caspases: valine-alanine-aspartic acid (VAD) for poly-caspases (including caspase-1, -3, -4, -5, -6, -7, -8, and -9), and aspartic acid-glutamic acid-valine-aspartic acid (DEVD) for caspase 3/7. A sulforhodamine (SR) group or carboxyfluorescein (FAM) group is attached as a reporter.

The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining red or green fluorescent signal is a direct measure of the amount of active caspase present at the time the inhibitor was added. The approximate excitation and emission peaks of the FLICA, propidium iodide, and Hoechst 33342 reagents are 488⁄ nm/530 nm, 535⁄ nm/6617 nm, and 350 nm/⁄461 nm, respectively. The detection reagents included within the Image-iT LIVE Red Poly Caspases, Red Caspase-3/7, Green Poly Caspases, and Green Caspase-3/7 Detection kits are SR-VAD-FMK, SR-DEVD-FMK, FAM-VAD-FMK, and FAM-DEVD-FMK, respectively.
For Research Use Only. Not for use in diagnostic procedures.

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