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Bethyl Laboratories
Phospho-gamma-H2AX (Ser139) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-gamma-H2AX (Ser139) Antibody (IHC-00059-T) in IHC (P)
Detection of mouse gamma-H2AX by Immunohistochemistry. Sample: FFPE section of mouse renal call carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX (Product # IHC-00059) (lot 9) used at a dilution of 1:500. Detection: DAB.
Product Details
IHC-00059-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region surrounding phosphorylated serine 139 of human histone H2AX.
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Amount
1 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig_10141, Pig, Monkey, Gorilla, Little brown bat, hirteen-lined ground squirrel and Chinese hamster
Target Information
H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax; H2A histone family member X; H2a/x; H2AFX; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2AX; unnamed protein product
Gene Aliases: AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax
UniProt ID: (Human) P16104, (Mouse) P27661
Entrez Gene ID: (Human) 3014, (Mouse) 15270
DNA damage checkpoint
double-strand break repair via homologous recombination
DNA repair
double-strand break repair
DNA recombination
nucleosome assembly
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
heterochromatin assembly
positive regulation of DNA repair
meiotic cell cycle
protein K63-linked ubiquitination
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
cell cycle
cellular response to gamma radiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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