Anza™ 10-Pack Starter Kit

Catalog number: IVGN3006
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Anza™ 10-Pack Starter Kit

Catalog number: IVGN3006
Have Questions?
Catalog Number
Unit Size
1 kit
Price (USD)
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
IVGN30061 kit
Product Overview
Citations & References
Additional Information
The Invitrogen™ Anza™ 10-Pack Starter Kit includes the 10 common restriction enzymes, 2 DNA modifying enzymes, and buffers listed below:

• Anza™ 11 EcoRI
• Anza™ 5 BamHI
• Anza™ 1 NotI
• Anza™ 16 HindIII
• Anza™ 12 XbaI
• Anza™ 8 XhoI
• Anza™ 3 BcuI (isoschizomer to SpeI)
• Anza™ 10 DpnI
• Anza™ 19 BglII
• Anza™ 26 Eco32I (isoschizomer to EcoRV)
• Anza™ T4 DNA Ligase Master Mix
• Anza™ Alkaline Phosphatase
• Anza™ Buffer (10X)
• Anza™ Red Buffer (10X)

For a kit with just 5 enzymes, see the Anza™ 5-Pack Starter Kit.

The Anza™ Restriction Enzyme Cloning System employs a single buffer and protocol for all Anza restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This eliminates the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.

The Anza 10-pk Starter Kit is a subset of the Anza Restriction Enzyme Cloning System, unifying the traditional cloning processes. For more information on the system, visit
For Research Use Only. Not for use in diagnostic procedures.


Shipping Condition
Approved for shipment on Wet or Dry Ice
Methylation Sensitivity
Methylation Sensitivities Varies by Enzyme
HindIII, NotI, Alkaline Phosphatase, XhoI, BcuI, XbaI, DpnI, T4 DNA Ligase, Eco32I, BamHI, BglII, EcoRI
Compatible Buffer
Anza™ 10X Red Buffer, Anza™ 10X Buffer
Product Type
Starter Kit
Research Category
Traditional Cloning
Product Line

Contents & Storage

20 μL each of Anza 11 EcoRI, Anza 5 BamHI, Anza 1 NotI, Anza 16 HindIII, Anza 12 XbaI
20 μL each of Anza 3 BcuI, Anza 8 XhoI, Anza 10 DpnI, Anza 19 BglII, Anza 26 Eco32I
100 μL Anza T4 DNA Ligase Master Mix
20 μL Anza Alkaline Phosphatase
500 μL Anza Buffer (10X)
500 μL Anza Red Buffer (10X)

Store at -5 to -30°C.


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    Frequently asked questions (FAQs)

    Citations & References

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