Invitrogen™

Anza™ 10-Pack Starter Kit

Catalog number:  IVGN3006

 Related applications:

Restriction Enzyme Cloning

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Description

The Invitrogen™ Anza™ 10-Pack Starter Kit includes the 10 common restriction enzymes, 2 DNA modifying enzymes, and buffers listed below:

• Anza™ 11 EcoRI
• Anza™ 5 BamHI
• Anza™ 1 NotI
• Anza™ 16 HindIII
• Anza™ 12 XbaI
• Anza™ 8 XhoI
• Anza™ 3 BcuI (isoschizomer to SpeI)
• Anza™ 10 DpnI
• Anza™ 19 BglII
• Anza™ 26 Eco32I (isoschizomer to EcoRV)
• Anza™ T4 DNA Ligase Master Mix
• Anza™ Alkaline Phosphatase
• Anza™ Buffer (10X)
• Anza™ Red Buffer (10X)

For a kit with just 5 enzymes, see the Anza™ 5-Pack Starter Kit.

The Anza™ Restriction Enzyme Cloning System employs a single buffer and protocol for all Anza restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This eliminates the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.

The Anza 10-pk Starter Kit is a subset of the Anza Restriction Enzyme Cloning System, unifying the traditional cloning processes. For more information on the system, visit www.thermofisher.com/anza.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Compatible Buffer: Anza™ 10x Buffer, Anza™ 10x Red Buffer
Enzyme: Alkaline Phosphatase, BamHI, BcuI, BglII, DpnI, Eco32I, EcoRI, HindIII, NotI, T4 DNA Ligase, XbaI, XhoI
Methylation Sensitivity: Methylation sensitivities varies by enzyme. Check enzyme insert for specifics.
Product Line: Anza™
Product Size: 1 kit
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

20 µL each of Anza 11 EcoRI, Anza 5 BamHI, Anza 1 NotI, Anza 16 HindIII, Anza 12 XbaI
20 µL each of Anza 3 BcuI, Anza 8 XhoI, Anza 10 DpnI, Anza 19 BglII, Anza 26 Eco32I
100 µL Anza T4 DNA Ligase Master Mix
20 µL Anza Alkaline Phosphatase
500 µL Anza Buffer (10X)
500 µL Anza Red Buffer (10X)

Store at -5 to -30°C.

Documents

Manuals & protocols