Protease Inhibitor Cocktail II

Protease Inhibitor Cocktail II is recommended for use with bacterial cell extracts. We recommend a 5 mL stock solution of Protease Inhibitor Cocktail II for the inhibition of proteases extracted from 20 g of a bacterial pellet.

When reconstituted, each vial of Protease Inhibitor Cocktail II contains the following components:

• 20 mM AEBSF.HCl
• 1.7 mM Bestatin
• 200 µM E-64
• 85 mM EDTA
• 2 mM Pepstatin A

Protease Inhibitor Cocktail II is recommended for use with bacterial cell extracts. You should first reconstitute each vial in 1 mL DMSO. Then add 4 mL DI water to obtain a 5 mL stock solution. The solution may appear turbid. The 5 mL stock is enough to inhibit protease activity extracted from a 20 g bacterial cell pellet.

After reconstitution, we recommend that you aliquot and freeze the solution at -20°C. Stock solutions are stable for at least 1 month at -20°C.

If the solution appears turbid after reconstitution, the solution for this material will appear turbid throughout its usage. This is normal and the material will function without issue.