Thermo Scientific™

Maxima Probe/ROX qPCR Master Mix (2X)

Catalog number: K0231
Thermo Scientific™

Maxima Probe/ROX qPCR Master Mix (2X)

Catalog number: K0231
Catalog Number
K0231
Unit Size
200 reactions
Price (USD)
217.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 reactions
Price (USD)
894.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4,000 reactions
Price (USD)
2,530.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0231200 reactions
217.00
Your Price:
***
K02321,000 reactions
894.00
Your Price:
***
K02334,000 reactions
2,530.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Specifications

Concentration
2X
Detection Method
Primer-Probe Detection
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
200 reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
Volume
2 x 1.25 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line
Maxima
Product Type
qPCR Master Mix
Sufficient For
200 Reactions of 25 μL

Contents & Storage

Contains:
• 2 x 1.25 mL Maxima Probe/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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