Mezcla maestra de qPCR Maxima Probe (2X), con vial ROX independiente
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Thermo Scientific™

Mezcla maestra de qPCR Maxima Probe (2X), con vial ROX independiente

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’veMás información
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Número de catálogoCantidad
K02634,000 reactions
Número de catálogo K0263
Precio (CLP)
3.008.997
Each
Añadir al carro de la compra
Cantidad:
4,000 reactions
Precio (CLP)
3.008.997
Each
Añadir al carro de la compra
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe qPCR Master Mix, ROX Solution provided (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (equipo)BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
FormatoTubo
N.º de reacciones4000 reacciones
PolimerasaADN polimerasa Taq
Línea de productosMaxima
Tipo de productoqPCR Master Mix
Cantidad4,000 reactions
Tipo de muestraADN, ADNbc
Suficiente para4000 reacciones de 25 μl
Volumen4 x 12.5 mL
Concentración2X
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Gene Expression, Genotyping, siRNA Validation
GC-Rich PCR PerformanceHigh
Método de PCRqPCR
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Contains:
• 4 x 12.5 mL Maxima Probe qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer; sufficient for 4000 x 25 μL reactions.
• 1 mL ROX solution, 50 μM
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.