Biochemical characterization of the human ubiquitous glucose-6-phosphatase in neutrophil granulocytes.
AuthorsLédeczi Z,Németh K,Kardon T
JournalFEBS open bio
PubMed ID39548727
Glucose‐6‐phosphatase‐β (G6PC3) is a ubiquitous phosphatase present in the endoplasmic reticulum, which, unlike G6PC1, is not responsible for maintaining blood glucose level under starvation. Recently, G6PC3 has been shown to play an important role in neutrophil granulocytes, eliminating the toxic metabolite 1,5‐anhydroglucitol‐6‐phosphate. The present study aimed to look for alternative ... More
Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1.
AuthorsNashed A,Naidoo KJ
JournalACS omega
PubMed ID38645360
[Image: see text] Chemical systems glycobiology requires experimental and computational tools to make possible big data analytics benefiting genomics and proteomics. The impediment to tool development is that the nature of glycan construction and mutation is not template driven but rests on cooperative glycosyltransferase (GT) catalytic synthesis. What is needed ... More
Inhibition of a lower potency target drives the anticancer activity of a clinical p38 inhibitor.
The small-molecule drug ralimetinib was developed as an inhibitor of the p38α mitogen-activated protein kinase, and it has advanced to phase 2 clinical trials in oncology. Here, we demonstrate that ralimetinib resembles EGFR-targeting drugs in pharmacogenomic profiling experiments and that ralimetinib inhibits EGFR kinase activity in vitro and in cellulo. ... More
Low dietary fiber intake impairs small intestinal Th17 and intraepithelial T cell development over generations.
AuthorsRoyer CJ,Rodriguez-Marino N,Yaceczko MD,Rivera-Rodriguez DE,Ziegler TR,Cervantes-Barragan L
JournalCell reports
PubMed ID37768824
Dietary fiber strongly impacts the microbiota. Here, we show that a low-fiber diet changes the small intestinal (SI) microbiota and impairs SI Th17, TCRαβ( )CD8αβ( ) and TCRαβ( )CD8αα( ) intraepithelial T cell development. We restore T cell development with dietary fiber supplementation, but this defect becomes persistent over generations ... More