NovaFluor™ Antibody Conjugation Kits
NovaFluor™ Antibody Conjugation Kits
NovaFluor™ Antibody Conjugation Kits
NovaFluor™ Antibody Conjugation Kits
Invitrogen™

NovaFluor™ Antibody Conjugation Kits

Get the most out of your panel design by conjugating antibodies to NovaFluors: dyes specifically designed for spectral hygiene to have narrow emission spectra and minimal cross-laser excitation.
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Catalog NumberLabel or DyeProduct TypeEmissionQuantity
K06T04L013NovaFluor™ Yellow 590Conjugation Kit590 nm100 μg
K06T04L008NovaFluor™ Blue 510Conjugation Kit510 nm100 μg
K06T04L002NovaFluor™ Blue 530Conjugation Kit530 nm100 μg
K06T04L009NovaFluor™ Blue 555Conjugation Kit555 nm100 μg
K06T04L010NovaFluor™ Blue 585Conjugation Kit585 nm100 μg
K06T04L003NovaFluor™ Blue 610/30SConjugation Kit610 nm100 μg
K06T04L011NovaFluor™ Blue 610/70SConjugation Kit610 nm100 μg
K06T04L004NovaFluor™ Blue 660/40SConjugation Kit660 nm100 μg
K06T04L021NovaFluor™ Blue 690Conjugation Kit690 nm100 μg
K06T04L012NovaFluor™ Blue 660/120SConjugation Kit660 nm100 μg
K06T04L026NovaFluor™ Blue 760Conjugation Kit760 nm100 μg
K06T04L017NovaFluor™ Red 660Conjugation Kit660 nm100 μg
K06T04L018NovaFluor™ Red 685Conjugation Kit685 nm100 μg
K06T04L019NovaFluor™ Red 700Conjugation Kit700 nm100 μg
K06T04L020NovaFluor™ Red 710Conjugation Kit710 nm100 μg
K06T04L023NovaFluor™ Red 725Conjugation Kit725 nm100 μg
K06T04L024NovaFluor™ Red 755Conjugation Kit755 nm100 μg
K06T04L005NovaFluor™ Yellow 570Conjugation Kit570 nm100 μg
K06T04L006NovaFluor™ Yellow 610Conjugation Kit610 nm100 μg
K06T04L007NovaFluor™ Yellow 660Conjugation Kit660 nm100 μg
K06T04L014NovaFluor™ Yellow 690Conjugation Kit690 nm100 μg
K06T04L015NovaFluor™ Yellow 700Conjugation Kit700 nm100 μg
K06T04L016NovaFluor™ Yellow 730Conjugation Kit730 nm100 μg
K06T04L022NovaFluor™ Yellow 755Conjugation Kit755 nm100 μg
K06T04L027NovaFluor™ UV 765Conjugation Kit765 nm100 μg
K06T04L028NovaFluor™ Violet 800Conjugation Kit800 nm100 μg
K06T04L029NovaFluor™ Violet 745Conjugation Kit745 nm100 μg
K06T04L030NovaFluor™ Yellow 810Conjugation Kit810 nm100 μg
K06T04L031NovaFluor™ Blue 725Conjugation Kit725 nm100 μg
K06T04L032NovaFluor™ Violet 690Conjugation Kit690 nm100 μg
K06T04L033NovaFluor™ Blue 800Conjugation Kit800 nm100 μg
Catalog number K06T04L013
Price (USD)
550.00
Each
Add to cart
Label or Dye:
NovaFluor™ Yellow 590
Product Type:
Conjugation Kit
Emission:
590 nm
Quantity:
100 μg
Price (USD)
550.00
Each
Add to cart
NovaFluor Conjugation Kits provide a simple workflow to conjugate a blue, yellow-green or red excitable fluorophore to your antibody of choice in just 3 easy steps. The fluorescence-by-design Phiton platform enables the addition of parameters to your current panels in discovery research and resolves stained populations with near-zero cross excitation, with a lack of emission in the violet channels that plague other fluorophores.

NovaFluor Conjugation Kit provides features including:

  • A clean and bright antibody label to increase the number of parameters on conventional and spectral flow cytometers
  • Resolve populations with limited/near-zero cross excitation and the lowest spread performance of any blue, yellow-green or red excited fluorescent labels
  • Simple 3-step protocol, with 60 sec. of hands-on time for conjugating 10–100 μg of antibody per label
  • Up to 70% less excitation when compared to PE tandems, resulting in decreased spillover and higher resolution

3-step conjugation method

  • Attach NovaFluor Linker to antibody of choice
  • Remove excess NovaFluor Linker from DNA-conjugate antibody
  • Anneal NovaFluor to oligo-conjugated antibody
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Emission590 nm
For Use With (Application)Flow Cytometry
Label or DyeNovaFluor™ Yellow 590
Product TypeConjugation Kit
Quantity100 μg
Product LineNovaFluor™
Unit SizeEach

Frequently asked questions (FAQs)

What is the NovaFluor dye structure and how does this impact the NovaFluor antibody conjugation workflow?

NovaFluor dyes are based on Phiton technology utilizing novel nucleic acid dye structures. The dyes work with FRET technology and have a clear benefit versus tandem dyes, as the structures are extremely stable. More information can be found on our NovaFluor website.

Due to the nucleotide structure, some adherence of the NovaFluor dyes to dead cells is expected. Good viability of the sample and inclusion of a fixable viability stain is recommended. Using nucleotide-staining viability dyes such as DAPI, PI, and 7-AAD dyes is not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use NovaFluor antibody conjugation kits for conjugation of antibodies targeting intracellular epitopes?

NovaFluor antibody conjugation kits can be used for conjugation of antibodies targeting intracellular epitopes. The labeling needs to be performed with 10 µL CellBlox Plus Blocking Buffer (Cat. No. C001T02F01, C001T03F01, C001T06F01) to block non-specific binding of NovaFluor labels with cells, after fixation and permeabilization.

If NovaFluor dyes are used for surface targets as well as intracellular targets, first add 5 µL CellBlox Plus Blocking Buffer during staining with the surface markers. Then, after completing the staining, washing, fixation, and permeabilization, add 10 µL CellBlox Plus Blocking Buffer again to block non-specific intracellular binding while labeling with the NovaFluor conjugates for intracellular targets.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I label my antibody with more than one NovaFluor label if I purchased more than one NovaFluor conjugation kit?

Yes, you can. After performing the steps on Days 1 and 2, your Antibody-NovaFluor Linker conjugate can be attached to the NovaFluor label at whatever volume you like, as long as the ratio of the Antibody-NovaFluor Linker to the NovaFluor label is kept constant.

Example: If you purchased two NovaFluor conjugation kits, one with NovaFluor Blue 610-30S and one with NovaFluor Yellow 610, use the contents of both kits to modify your two antibodies of interest (both 100 µg), completing all steps outlined for Days 1 and 2 with no modification. After resuspending your antibodies into PBS and after the second round of ammonium sulfate precipitation, you should have 100 µL of each Antibody-NovaFluor Linker conjugate. You can now divide the antibody solutions in half (50 µL each) and add half of each NovaFluor label to the divided antibody solutions (150 µL of NovaFluor label to each 50 µL of Antibody-NovaFluor Linker), giving you 200 µL total of each antibody conjugated to both NovaFluor Blue 610-30S and NovaFluor Yellow 610. After mixing, continue with the protocol and incubate overnight at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store NovaFluor-antibody conjugates?

We recommend storing new NovaFluor-antibody conjugates at 2-8 degrees C, protected from light. If desired, NovaFluor-antibody conjugates can be stored with 0.09% sodium azide. DO NOT store NovaFluor-antibody conjugates at -80 degrees C or -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the NovaFluor conjugation kits, in Step 16 of the procedure, can you provide more information about how I should orient my tube and describe the appearance of the pellet following precipitation?

We have included Figure 4 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025061_NovaFluorConjugationKit_UG.pdf) to show how the pellet appears following the precipitation step. Although the microcentrifuge tube can be centrifuged in any orientation, we recommend orienting it in the same direction for both centrifugation steps so that your pellet is in the same place (Figure 3). The pellet is often smaller after the second precipitation, so it is helpful to know where to expect it.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.