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Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Highlights
• Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 5 minutes
• Convenient: spin columns are capped and assembled with collection tubes
Applications
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In vitro transcription
• In situ hybridization
• Labeling
• Cloning
Format | Spin Column |
Elution Volume Max | 50 μL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Purification Time | 5 min. |
Quantity | 250 Preps |
Run Time | 5 min. |
Shipping Condition | Room Temperature |
Starting Material Volume | ≤400 μL |
Yield | 25 μg (Binding capacity) |
For Use With (Application) | Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization |
Final Product Type | DNA |
Isolation Technology | Silica Spin Column |
Sample Type | PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions) |
Unit Size | 250 preps |
Format | Spin Column |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Product Line | GeneJET |
Product Type | PCR Purification Kit |
Quantity | 50 Preps |
Run Time | 5 min. |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
For Use With (Application) | Real Time PCR (qPCR), Cloning, DNA Sequencing |
Isolation Technology | Spin Column |
Sample Type | dsDNA |
Unit Size | 50 preps |
Catalog Number | Specifications | Unit Size | Quantity | Price (USD) | Availability | Quantity | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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K0702 | 250 preps | 250 Preps | Price: 434.00 Online price: 372.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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K0701 | 50 preps | 50 Preps | Price: 98.50 Online price: 83.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Highlights
• Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 5 minutes
• Convenient: spin columns are capped and assembled with collection tubes
Applications
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In vitro transcription
• In situ hybridization
• Labeling
• Cloning