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Thermo Scientific™

GeneJET PCR Purification Kit

Catalog number: K0702
Thermo Scientific™

GeneJET PCR Purification Kit

Catalog number: K0702

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights
Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
Fast: procedure takes only 5 minutes
Convenient: spin columns are capped and assembled with collection tubes

Applications
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
In vitro transcription
In situ hybridization
• Labeling
• Cloning

 
Catalog Number
K0702
Unit Size
250 preps
Quantity
250 Preps
Price (USD)
Price: 434.00
Online price: 372.65
Your price:
Availability
-
Full specifications
Quantity
FormatSpin Column
Elution Volume Max50 μL
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time5 min.
Quantity250 Preps
Run Time5 min.
Shipping ConditionRoom Temperature
Starting Material Volume≤400 μL
Yield25 μg (Binding capacity)
For Use With (Application)Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization
Final Product TypeDNA
Isolation TechnologySilica Spin Column
Sample TypePCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Unit Size250 preps
Catalog Number
Unit Size
50 preps
Quantity
50 Preps
Price (USD)
Price: 98.50
Online price: 83.65
Your price:
Availability
-
Full specifications
Quantity
FormatSpin Column
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineGeneJET
Product TypePCR Purification Kit
Quantity50 Preps
Run Time5 min.
TargetDNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)Real Time PCR (qPCR), Cloning, DNA Sequencing
Isolation TechnologySpin Column
Sample TypedsDNA
Unit Size50 preps
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)AvailabilityQuantity
K0702Full specifications
250 preps250 Preps
Price: 434.00
Online price: 372.65
Your price:
-
FormatSpin Column
Elution Volume Max50 μL
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time5 min.
Quantity250 Preps
Run Time5 min.
Shipping ConditionRoom Temperature
Starting Material Volume≤400 μL
Yield25 μg (Binding capacity)
For Use With (Application)Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization
Final Product TypeDNA
Isolation TechnologySilica Spin Column
Sample TypePCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Unit Size250 preps
K0701Full specifications
50 preps50 Preps
Price: 98.50
Online price: 83.65
Your price:
-
FormatSpin Column
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineGeneJET
Product TypePCR Purification Kit
Quantity50 Preps
Run Time5 min.
TargetDNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)Real Time PCR (qPCR), Cloning, DNA Sequencing
Isolation TechnologySpin Column
Sample TypedsDNA
Unit Size50 preps
Showing 2 of 2

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights
Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
Fast: procedure takes only 5 minutes
Convenient: spin columns are capped and assembled with collection tubes

Applications
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
In vitro transcription
In situ hybridization
• Labeling
• Cloning

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• 60 mL Binding Buffer; room temperature
• 45 mL Wash Buffer (concentrated); room temperature
• 30 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature

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