Thermo Scientific™

      Rapid DNA Ligation Kit

      Rapid DNA Ligation Kit

      Catalog number:  K1422

       Related applications: PCR Cloning | Restriction Enzyme Cloning

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      Description

      Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

      Highlights

      • Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
      • Convenient—reaction mixture can be used directly for bacterial transformation

      Applications

      • Routine cloning experiments
      • Blunt-end cloning
      • Library construction
      • TA cloning

      Includes

      • T4 DNA Ligase
      • 5X Rapid Ligation Buffer
      • Water, nuclease-free
      • Detailed Protocol

      Note

      Prior to electroporation, it is necessary to inactivate T4 DNA ligase by chloroform extraction or spin column purification, e.g. with Thermo Scientific GeneJET PCR Purification Kit (K0701).

      Related Products
      Rapid DNA Ligation Kit
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Enzyme: DNA Ligase
      Product Size: 50 reactions

      Documents

      Manuals & protocols

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