Thermo Scientific™

Rapid DNA Ligation Kit

Catalog number:  K1422

 Related applications:

PCR Cloning

| Restriction Enzyme Cloning

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Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.


• Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
• Convenient—reaction mixture can be used directly for bacterial transformation


• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloning


T4 DNA Ligase
• 5X Rapid Ligation Buffer
Water, nuclease-free
• Detailed Protocol


Prior to electroporation, it is necessary to inactivate T4 DNA ligase by chloroform extraction or spin column purification, e.g. with Thermo Scientific GeneJET PCR Purification Kit (K0701).

Related Products
Rapid DNA Ligation Kit
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: DNA Ligase
Product Size: 50 reactions


Manuals & protocols