Search

  • 주문 현황
  • 빠른주문

RiboMinus™ Concentration Module - FAQs

View additional product information for RiboMinus™ Concentration Module - FAQs (K155005)

2 product FAQs found

I'm getting low RNA yield after using a RiboMinus kit for RNA extraction. What could be the cause of this?

Low RNA content or a loss of the pellet during ethanol precipitation could lead to a low RNA yield. Additionally, various tissues have different RNA content, and the yield is dependent on the sample.

What is the best way to analyze RNA that I've isolated using a RiboMinus kit?

The purified RNA is easily quantitated using UV absorbance at 260 nm or a Quant-iT RNA Assay Kit. To verify rRNA depletion, electrophorese your sample on an agarose gel or use an Agilent 2100 Bioanalyzer. Agarose gel electrophoresis should show depletion of 18S and 28S rRNA bands compared to a control sample. Absence of contaminating DNA and RNA degradation may also be confirmed by agarose gel electrophoresis. The efficiency of rRNA depletion in the purified RNA, RNA degradation, and RNA concentration can also be analyzed using a bioanalyzer.