CellSensor™ c-Fos-bla ME-180 Cell Line
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Thermo Scientific™
CellSensor™ c-Fos-bla ME-180 Cell Line
The CellSensor™ c-fos-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the c-fos response element stably integratedRead more
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Catalog number K1650
Price (USD)/ 1 vial
10,580.00
-
Add to cart
Price (USD)/ 1 vial
10,580.00
Add to cart
CellSensor™ c-Fos-bla ME-180 Cell Line
Catalog numberK1650
Price (USD)/ 1 vial
10,580.00
-
Add to cart

The CellSensor™ c-fos-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the c-fos response element stably integrated into ME-180 cells. To construct this cell line, the c-fos-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to epidermal growth factor (EGF). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z' and EC50 concentrations for EGF. The CellSensor™ c-fos-bla ME-180 cell line is responsive to EGF, HGF, IL-6, OSM, TNFα, IL-1-alpha; and PMA/Thaps, and can be used to probe the JNK/P38/MAPK and JAK/STAT signaling pathways. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay EntryCell-based beta lactamase reporter gene
Cell StateDividing Cells
Product LineCellSensor™
Quantity1 Vial
Target EntrySTAT1, STAT3, JAK1, EGFR, ERK1/2, p38, MAPK pathway, RAF, JAK2, c-MET, JNK, JAK/STAT (IFN gamma, IL-6/OSM) pathway, RON, MEK
System TypeCellSensor™
Druggable TargetSignaling Pathway
Unit Size1 vial
Contents & Storage
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.