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Thermo Scientific™

Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase

Catalog number: K1671
Thermo Scientific™

Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase

Catalog number: K1671
Catalog Number
K1671
Unit Size
50 reactions
Price (USD)
392.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200 reactions
Price (USD)
1,280.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K167150 reactions
392.00
Your Price:
***
K1672200 reactions
1,280.00
Your Price:
***
Product Overview
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Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. It provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

For the first strand cDNA synthesis the kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT. The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C). The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

Highlights

• Integrated gDNA removal step
• High yields of full length cDNA up to 20 kb.
• Efficient cDNA synthesis at wide temperature range from 42°C to 65°C.
• Increased synthesis rate—complete cDNA synthesis in 15-30 minutes.
• High sensitivity and specificity.

Applications

• 2-step RT-PCR
• 2-step RT-qPCR

Maxima First Strand cDNA Synthesis Kit with dsDNase contains
• Maxima Enzyme Mix
• 5X Reaction Mix
• dsDNase
• 10X dsDNase Buffer
• nuclease-free water

Additional Information about Reaction Components
The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and Thermo Scientific RiboLock RNase Inhibitor,. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.

5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers.

Water, nuclease-free is provided for reaction set-up and dilution of sample RNA. The absence of endo-, exodeoxyribonucleases, ribonucleases and phosphatases has been confirmed by appropriate quality tests.

Related Products
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima First Strand cDNA Synthesis Kit Reaction Mix (5X)

Specifications

Format
Tube
No. of Reactions
50 x 20 μL Reactions
PCR Method
2-Step RT-qPCR
Reaction Format
Separate Components
Sample Type
RNA
For Use With (Application)
Real-Time Quantitative PCR (qPCR)
Optimal Reaction Temperature
50-55° C
Product Line
Maxima
Product Type
First Strand CDNA Synthesis Kit
Quantity
50 x 20μL Reactions
Reverse Transcriptase
Maxima
Sufficient For
50 x 20 μL Reactions

Contents & Storage

Maxima First Strand cDNA Synthesis Kit with dsDNase contains:
• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers)
• dsDNase
• 10X dsDNase Buffer
• nuclease-free water

Store at -20°C.

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