LanthaScreen™ c-Jun (1-79) HeLa Cell Line

The LanthaScreen™ c-Jun (1-79) HeLa cell line contains a fusion protein consisting of GFP and a fragment encoding for AA 1-79 of c-Jun under the control of a CMV promoter stably transfected into HeLa cell lines. HeLa is a cervical cancer cell line which shows inducible activation of JNK by a number of different ligands, such as TNF-a and EGF, which leads to the transient phosphorylation of GFP-c-Jun (1-79). This LanthaScreen™ cell line therefore allows the analysis of JNK activity using GFP-c-Jun phosphorylation as readout. The GFP-c-Jun (1-79) construct was transfected into HeLa cells using Lipofectamine™ 2000, followed by selection with Blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF-a as ligand for JNK mediated GFP-c-Jun (1-79) phosphorylation. This cell lines has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time. Additional information using alternate stimuli is also available.

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