During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.

LanthaScreen® c-Jun (1-79) HeLa Cell Line

Catalog number: K1680

 Related applications: Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The LanthaScreen®c-Jun (1-79) HeLa cell line contains a fusion protein consisting of GFP and a fragment encoding for AA 1-79 of c-Jun under the control of a CMV promoter stably transfected into HeLa cell lines. HeLa is a cervical cancer cell line which shows inducible activation of JNK by a number of different ligands, such as TNF-a and EGF, which leads to the transient phosphorylation of GFP-c-Jun (1-79). This LanthaScreen®cell line therefore allows the analysis of JNK activity using GFP-c-Jun phosphorylation as readout. The GFP-c-Jun (1-79) construct was transfected into HeLa cells using Lipofectamine™2000, followed by selection with Blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF-a as ligand for JNK mediated GFP-c-Jun (1-79) phosphorylation. This cell lines has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time. Additional information using alternate stimuli is also available. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: LanthaScreen®
Cell State: Dividing Cells
Product Size: 1 vial
Druggable Target: Kinases, Signaling Pathway

Contents & storage

LanthaScreen® Cellular assays are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

Documents

Manuals & protocols