Thermo Scientific™

CellSensor™ ESRE-bla HeLa Cell Line

Catalog number: K1817
Thermo Scientific™

CellSensor™ ESRE-bla HeLa Cell Line

Catalog number: K1817
Catalog Number
K1817
Unit Size
1 vial
Price (USD)
Price: 13,180.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K18171 vial
Price: 13,180.00
Your Price:
***
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Endoplasmic Reticulum (ER) stress is associated with a variety of pathophysiological conditions, such as neurodegenerative diseases, diabetes, tumor growth under hypoxic conditions, and ischemic heart disease. Proteins in the ER misfold or unfold, and accumulate under stress conditions, which promote the expression of ER stress responsive genes. One of the mechanisms for mediating ER stress response is the activation of transcription factor ATF6. The quiescent form of ATF6 (p90ATF6), a type II-transmembrane protein, is embedded in the ER membrane and proteolyzed in an ER stress-dependent manner. The liberated N-terminal fragment (p50ATF6) translocates to the nucleus, binding to ER stress response element (ERSE) present in the proximal promoter regions of many ER stress-responsive proteins including ER chaperones. To better understand the pathological processes and provide novel avenues to potential therapies, ESRE–bla HeLa cells are engineered to express beta-lactamase under the control of ER stress response element. This is a clonal population isolated by FACS and its dose response curves with tunicamycin and thapsigargin are performed. This cell line also response to other known ER stress inducers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Thapsigargin
Antagonists
Salubrinal
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
ESRE
Human Protein Class
Cell Signalling
Primary Agonist
Tunicamycin
Product Line
CellSensor™
Readout
End Point
Reporter Gene
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Target Entry
ATF6, ER stress response pathway
Cell Line
HeLa
System Type
CellSensor™
Druggable Target
Signaling Pathway

Contents & Storage

CellSensor™ ESRE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

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