Contribution of acquired carbapenem-hydrolyzing oxacillinases to carbapenem resistance in Acinetobacter baumannii.
AuthorsHéritier C, Poirel L, Lambert T, Nordmann P
JournalAntimicrob Agents Chemother
PubMed ID16048925
'Carbapenem-hydrolyzing oxacillinases are reported increasingly in Acinetobacter baumannii. Since they hydrolyze carbapenems at low levels, the roles of carbapenem-hydrolyzing oxacillinases OXA-23, OXA-40, and OXA-58 in A. baumannii were determined. The bla(OXA-23), bla(OXA-40), and bla(OXA-58) genes were inserted in broad-host-range plasmid pAT801 and transformed in Escherichia coli DH10B and in A. ... More
The origin recognition complex marks a replication origin in the human TOP1 gene promoter.
'The locations of the origin recognition complex (ORC) in mammalian genomes have been elusive. We have therefore analyzed the DNA sequences associated with human ORC via in vivo cross-linking and chromatin immunoprecipitation. Antibodies specific for hOrc2 protein precipitate chromatin fragments that also contain other ORC proteins, suggesting that the proteins ... More
De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1.
Authors Chalfant Charles E; Rathman Kristin; Pinkerman Ryan L; Wood Rachel E; Obeid Lina M; Ogretmen Besim; Hannun Yusuf A;
JournalJ Biol Chem
PubMed ID11801602
'In a recent study, we showed that ceramide induces the dephosphorylation of SR proteins, a family of protein factors that regulate alternative splicing. In this study, the regulation of the alternative processing of pre-mRNA of both caspase 9 and Bcl-x(L) was examined in response to ceramide. Treatment of A549 lung ... More
A gene network for long-day flowering activates RFT1 encoding a mobile flowering signal in rice.
AuthorsKomiya R, Yokoi S, Shimamoto K
JournalDevelopment
PubMed ID19762423
'Although some genes that encode sensory or regulatory elements for photoperiodic flowering are conserved between the long-day (LD) plant Arabidopsis thaliana and the short-day (SD) plant rice, the gene networks that control rice flowering, and particularly flowering under LD conditions, are not well understood. We show here that RICE FLOWERING ... More
Estrogen receptor-mediated regulation of microRNA inhibits proliferation of vascular smooth muscle cells.
AuthorsZhao J, Imbrie GA, Baur WE, Iyer LK, Aronovitz MJ, Kershaw TB, Haselmann GM, Lu Q, Karas RH
JournalArterioscler Thromb Vasc Biol
PubMed ID23175673
Estradiol (E2) regulates gene transcription by activating estrogen receptor-a and estrogen receptor-ß. Many of the genes regulated by E2 via estrogen receptors are repressed, yet the molecular mechanisms that mediate E2-induced gene repression are currently unknown. We hypothesized that E2, acting through estrogen receptors, regulates expression of microRNAs (miRs) leading ... More
RAG2 is down-regulated by cytoplasmic sequestration and ubiquitin-dependent degradation.
AuthorsMizuta R, Mizuta M, Araki S, Kitamura D,
JournalJ Biol Chem
PubMed ID12205088
Periodic accumulation and degradation of RAG2 (recombination-activating gene 2) protein controls the cell-cycle-dependent V(D)J recombination of lymphocyte antigen receptor genes. Here we show the molecular mechanism of RAG2 degradation. The RAG2 protein is translocated from the nucleus to the cytoplasm and degraded through the ubiquitin/proteasome system. RAG2 translocation is mediated ... More
Crystal structure of conserved hypothetical protein Aq1575 from Aquifexaeolicus.
Authors Shin Dong Hae; Yokota Hisao; Kim Rosalind; Kim Sung-Hou;
JournalProc Natl Acad Sci U S A
PubMed ID12060744
The crystal structure of a conserved hypothetical protein, Aq1575, from Aquifex aeolicus has been determined by using x-ray crystallography. The protein belongs to the domain of unknown function DUF28 in the Pfam and PALI databases for which there was no structural information available until now. A structural homology search with ... More
Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas.
Authors Martin Alberto; Bardwell Philip D; Woo Caroline J; Fan Manxia; Shulman Marc J; Scharff Matthew D;
JournalNature
PubMed ID11823785
The production of high-affinity protective antibodies requires somatic hypermutation (SHM) of the antibody variable (V)-region genes. SHM is characterized by a high frequency of point mutations that occur only during the centroblast stage of B-cell differentiation. Activation-induced cytidine deaminase (AID), which is expressed specifically in germinal-centre centroblasts, is required for ... More
SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint.
Authors Yazdi Parvin T; Wang Yi; Zhao Song; Patel Nimitt; Lee Eva Y-H P; Qin Jun;
JournalGenes Dev
PubMed ID11877377
Structural maintenance of chromosomes (SMC) proteins (SMC1, SMC3) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. Here we report that SMC1 is a component of the DNA damage response network that functions as ... More
Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses.
AuthorsNoguchi K, Fukazawa H, Murakami Y, Uehara Y
JournalJ Biol Chem
PubMed ID12154088
DNA replication and genotoxic stresses activate various checkpoint-associated protein kinases, and checkpoint dysfunction often leads to cell lethality. Here, we have identified new members of the mammalian NIMA family of kinases, termed Nek11L and Nek11S (NIMA-related kinase 11 Long and Short isoform) as novel DNA replication/damage stresses-responsive kinases. Molecular cloning ... More
In Vivo Reconstitution of Saccharomyces cerevisiae DNA Polymerase epsilon in Insect Cells. PURIFICATION AND CHARACTERIZATION.
Authors Dua Rajiv; Levy Daniel L; Li Caroline M; Snow Peter M; Campbell Judith L;
JournalJ Biol Chem
PubMed ID11756442
DNA polymerase epsilon (pol epsilon) is a multiple subunit complex consisting of at least four proteins, including catalytic Pol2p, Dpb2p, Dpb3p, and Dpb4p. Pol epsilon has been shown to play essential roles in chromosomal DNA replication. Here, we report reconstitution of the yeast pol epsilon complex, which was expressed and ... More
Transmission of human immunodeficiency virus type 1 from a patient who developed AIDS to an elite suppressor.
AuthorsBailey JR, O'Connell K, Yang HC, Han Y, Xu J, Jilek B, Williams TM, Ray SC, Siliciano RF, Blankson JN
JournalJ Virol
PubMed ID18495769
Elite suppressors (ES) are untreated human immunodeficiency virus type 1 (HIV-1)-infected patients who maintain viral loads of <50 copies/ml. The mechanisms involved in this control of viral replication remain unclear. Prior studies suggested that these patients, as well as long-term nonprogressors, are infected with defective HIV-1 variants. Other reports have ... More
Malarial dihydroorotate dehydrogenase. Substrate and inhibitor specificity.
Authors Baldwin Jeffrey; Farajallah Azizeh M; Malmquist Nicholas A; Rathod Pradipsinh K; Phillips Margaret A;
JournalJ Biol Chem
PubMed ID12189151
The malarial parasite relies on de novo pyrimidine biosynthesis to maintain its pyrimidine pools, and unlike the human host cell it is unable to scavenge preformed pyrimidines. Dihydroorotate dehydrogenase (DHODH) catalyzes the oxidation of dihydroorotate (DHO) to produce orotate, a key step in pyrimidine biosynthesis. The enzyme is located in ... More