Molecular cloning and characterization of Foxp3 in Atlantic salmon (Salmo salar).
AuthorsZhang Z, Chi H, Niu C, Bøgwald J, Dalmo RA,
JournalFish Shellfish Immunol
PubMed ID21276855
'Foxp3 is a T cell-specific transcription factor and plays a key role in the development of Treg cells and in the immune regulatory process during inflammation. Here we report cloning and characterization of the full-length cDNA of Atlantic salmon Foxp3, which possesses a Forkhead domain, a zinc finger domain and ... More
Detection of cytosine methylation in RNA using bisulfite sequencing.
AuthorsPollex T, Hanna K, Schaefer M
JournalCold Spring Harb Protoc
PubMed ID20889702
'Post-transcriptional RNA modifications are a characteristic feature of noncoding RNAs and have been described for ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), and various other small RNAs. However, the biological function of most of these modifications remains uncharacterized. Cytosine-5 methylation (5mC) has been detected in abundant and long-lived RNA molecules such ... More
Unique DNA methylome profiles in CpG island methylator phenotype colon cancers.
AuthorsXu Y, Hu B, Choi AJ, Gopalan B, Lee BH, Kalady MF, Church JM, Ting AH
JournalGenome Res
PubMed ID21990380
'A subset of colorectal cancers was postulated to have the CpG island methylator phenotype (CIMP), a higher propensity for CpG island DNA methylation. The validity of CIMP, its molecular basis, and its prognostic value remain highly controversial. Using MBD-isolated genome sequencing, we mapped and compared genome-wide DNA methylation profiles of ... More
Enhanced autointegration in hyperstable simian immunodeficiency virus capsid mutants blocked after reverse transcription.
AuthorsTipper C, Sodroski J
JournalJ Virol
PubMed ID23345510
'After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replication defects ... More
Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.
AuthorsKoterba KL, Borgschulte T, Laird MW
JournalJ Biotechnol
PubMed ID22138638
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in ... More
Genome and transcriptome sequencing of lung cancers reveal diverse mutational and splicing events.
AuthorsLiu J, Lee W, Jiang Z, Chen Z, Jhunjhunwala S, Haverty PM, Gnad F, Guan Y, Gilbert HN, Stinson J, Klijn C, Guillory J, Bhatt D, Vartanian S, Walter K, Chan J, Holcomb T, Dijkgraaf P, Johnson S, Koeman J, Minna JD, Gazdar AF, Stern HM, Hoeflich KP, Wu TD, Settleman J, de Sauvage FJ, Gentleman RC, Neve RM, Stokoe D, Modrusan Z, Seshagiri S, Shames DS, Zhang Z
JournalGenome Res
PubMed ID23033341
Lung cancer is a highly heterogeneous disease in terms of both underlying genetic lesions and response to therapeutic treatments. We performed deep whole-genome sequencing and transcriptome sequencing on 19 lung cancer cell lines and three lung tumor/normal pairs. Overall, our data show that cell line models exhibit similar mutation spectra ... More