RIP140-targeted repression of gene expression in adipocytes.
AuthorsChristian M, Kiskinis E, Debevec D, Leonardsson G, White R, Parker MG,
JournalMol Cell Biol
PubMed ID16227589
'Ligand-dependent repression of nuclear receptor activity forms a novel mechanism for regulating gene expression. To investigate the intrinsic role of the corepressor RIP140, we have monitored gene expression profiles in cells that express or lack the RIP140 gene and that can be induced to undergo adipogenesis in vitro. In contrast ... More
Congenital Disorder of Glycosylation Id (CDG-Id) Presenting with Hyperinsulinemic Hypoglycemia and Islet Cell Hyperplasia.
'Context: Inborn errors in protein glycosylation such as the congenital disorders of glycosylation (CDG) generate multifaceted syndromes that impair many organ systems. We here report the diagnosis of the third known patient with CDG-Id. Results: The patient''s phenotype was extremely severe and she succumbed at nineteen days of age. Leading ... More
Group VIB Ca2+-independent Phospholipase A2{gamma} Promotes Cellular Membrane Hydrolysis and Prostaglandin Production in a Manner Distinct from Other Intracellular Phospholipases A2.
AuthorsMurakami M, Masuda S, Ueda-Semmyo K, Yoda E, Kuwata H, Takanezawa Y, Aoki J, Arai H, Sumimoto H, Ishikawa Y, Ishii T, Nakatani Y, Kudo I,
JournalJ Biol Chem
PubMed ID15695510
'Although group VIA Ca(2+)-independent phospholipase A(2)beta (iPLA(2)beta) has been implicated in various cellular events, the functions of other iPLA(2) isozymes remain largely elusive. In this study, we examined the cellular functions of group VIB iPLA(2)gamma. Lentiviral transfection of iPLA(2)gamma into HEK293 cells resulted in marked increases in spontaneous, stimulus-coupled, and ... More
A Novel Diacylglycerol-lactone Shows Marked Selectivity in Vitro among C1 Domains of Protein Kinase C (PKC) Isoforms {alpha} and {delta} as Well as Selectivity for RasGRP Compared with PKC{alpha}.
AuthorsPu Y, Perry NA, Yang D, Lewin NE, Kedei N, Braun DC, Choi SH, Blumberg PM, Garfield SH, Stone JC, Duan D, Marquez VE,
JournalJ Biol Chem
PubMed ID15923197
'Although multiple natural products are potent ligands for the diacylglycerol binding C1 domain of protein kinase C (PKC), RasGRP, and related targets, the high conservation of C1 domains has impeded the development of selective ligands. We characterized here a diacylglycerol-lactone, 130C037, emerging from a combinatorial chemical synthetic strategy, which showed ... More
Methionine sulfoxide reductase A is important for lens cell viability and resistance to oxidative stress.
Age-related cataract, an opacity of the eye lens, is the leading cause of visual impairment in the elderly, the etiology of which is related to oxidative stress damage. Oxidation of methionine to methionine sulfoxide is a major oxidative stress product that reaches levels as high as 60% in cataract while ... More
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
The endothelial cell (EC)-derived tissue inhibitor of metalloproteinase-2 (TIMP-2) and pericyte-derived TIMP-3 are shown to coregulate human capillary tube stabilization following EC-pericyte interactions through a combined ability to block EC tube morphogenesis and regression in three-dimensional collagen matrices. EC-pericyte interactions strongly induce TIMP-3 expression by pericytes, whereas ECs produce TIMP-2 ... More
P75 interacts with the Nogo receptor as a co-receptor for Nogo, MAG and OMgp.
AuthorsWang KC, Kim JA, Sivasankaran R, Segal R, He Z,
JournalNature
PubMed ID12422217
In inhibiting neurite outgrowth, several myelin components, including the extracellular domain of Nogo-A (Nogo-66), oligodendrocyte myelin glycoprotein (OMgp) and myelin-associated glycoprotein (MAG), exert their effects through the same Nogo receptor (NgR). The glycosyl phosphatidylinositol (GPI)-anchored nature of NgR indicates the requirement for additional transmembrane protein(s) to transduce the inhibitory signals ... More