ViraPower™ Lentiviral Directional TOPO® Expression Kit

Catalog number: K495000

Invitrogen™  Related applications: Protein Expression

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Description

The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Quick convenient Directional TOPO® cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti6⁄V5-D™-TOPO® vector
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R70007)
• One Shot® Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄V5™ Directional TOPO® Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Gateway® Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit (K5310-00)
• pLenti6.3?V5™-TOPO® TA Cloning® Kit (K531520)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Directional TOPO®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: Directional TOPO Vectors
Constitutive or Inducible System: Constitutive
Product Size: 1 kit

Contents & storage

pLenti6⁄V5™ Directional TOPO® Cloning Kit:
• pLenti6⁄V5™ Directional TOPO® Reagents: -20°C
• One Shot® Stbl3™ Chemically Competent E. coli: -80°C

ViraPower™ Bsd Lentiviral Support Kit:
• ViraPower™ Packaging Mix: -20°C
• Lipofectamine® 2000: 4°C (DO NOT freeze)
• Blasticidin: -20°C
• 293FT Cell Line: liquid nitrogen

Documents

Manuals & protocols