Osteocalcin ELISA Kit, Human

Catalog number: KAQ1381

Novex™  Related applications: Protein Assays and Analysis

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Description

The Human Osteocalcin (OST) ELISA research-use-only kit is to be used for the quantitative determination of intact human osteocalcin in serum and plasma samples using 96-well plates and a microplate reader.

Performance characteristics
• Sensitivity: 0.08 ng/mL
• Standard curve range: 1.56‒75 ng/mL
• Sample types: serum, plasma
• Species cross-reactivity: human
• Sample volume: 25 μL
• Total assay incubation time: 2.5 hrs

Principle of the method
The Human Osteocalcin ELISA is a solid phase enzyme-amplified sensitivity immunoassay (EASIA) performed on a microtiter plate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of human osteocalcin. Standards and samples react with the capture monoclonal antibody (MAb 1) coated on the microtiter well and with a monoclonal antibody (MAb 2) labeled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich (coated MAb 1 - human osteocalcin - MAb 2 – HRP), the microtiter plate is washed to remove unbound enzyme-labeled antibody. Bound enzyme-labeled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB, ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance which is proportional to the human osteocalcin concentration. A standard curve is plotted and human osteocalcin concentration in a sample is determined by interpolation from the standard curve.

Background information
Osteocalcin or bone Gla protein (B.G.P.) is the major non-collagenous protein of the bone matrix. It has a molecular weight of 5,800 Da and contains 49 amino acids, including 3 residues of gamma carboxy-glutamic acid. Osteocalcin is synthesized in the bone by the osteoblasts. After production, it is partly incorporated in the bone matrix and the rest is found in the blood circulation. The exact physiological function of osteocalcin is still unclear. Studies show that the circulating levels of osteocalcin reflect the rate of bone formation.

Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: BGP, OC
Accession Number: NM_199173.4, NP_954642.1
Shipping Condition: Wet Ice
Marker: Osteocalcin
Gene ID: 632
Species: Human
Gene Symbol: BGLAP
Sensitivity: 0.4 ng/mL
Target Gene: bone gamma-carboxyglutamate (gla) protein
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 2-90 ng⁄ml
Sample Volume: 25 µl
Protein Family: Hormones & Receptors
Incubation Time: 2.5 hrs
Detection Method: Colorimetric
Research Category: Endocrinology, Signal Transduction
Sample Type (Specific): Plasma, Serum
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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