Amyloid beta 42 Ultrasensitive ELISA Kit, Human

Catalog number: KHB3544

Novex™  Related applications: Protein Assays and Analysis

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The Human Beta Amyloid [1-42] Ultrasensitive (Aβ42 US) ELISA research-use-only kit is to be used for the quantitative determination of Human Aβ42 in samples (e.g., cell culture supernatants, tissue homogenates, cerebrospinal fluid [CSF], etc.) using 96-well plates and a microplate reader. The assay recognizes both natural and synthetic forms of human Aβ42. The anti-human Aβ42 antibody used in this kit is capable of selectively detecting Aβ42 and not Aβ40/Aβ43.*

Performance characteristics
• Sensitivity: <1 pg/mL
• Standard curve range: 1.56–100 pg/mL
• Sample types: cell culture supernatants, tissue homogenates, cerebrospinal fluid (CSF)
• Species cross-reactivity: human
• Sample volume: 50 μL (prediluted)
• Total assay incubation time: 4 hrs

Principle of the method
The Human Aβ42 US kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus of human Aβ has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human Aβ content, control specimens and unknowns are pipetted into these wells, and co-incubated with a rabbit monoclonal antibody* specific for the COOH-terminus of the 1-42 Aβ sequence. This COOH-terminal sequence is created upon cleavage of the analyzed precursor.

Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After removal of excess anti-rabbit antibody, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human Aβ42 present in the original specimen.

Background information
Alzheimer’s Disease (AD) is characterized by the presence of extracellular plaques and intracellular neurofibrillary tangles (NFTs) in the brain. The major protein component of these plaques is β amyloid peptide (Aβ), a 40 to 43 amino acid peptide cleaved from amyloid precursor protein by β-secretase (i.e., BACE) and a putative γ (gamma) secretase. Increased release of the longer forms of Aβ peptide, Aβ42 or Aβ43, which have a greater tendency to aggregate than Aβ40, occurs in individuals expressing certain genetic mutations, expressing certain ApoE alleles, or may involve other, still undiscovered, factors. Many researchers theorize that it is this increased release of Aβ42/Aβ43 that leads to the abnormal deposition of Aβ and the associated neurotoxicity in the brains of affected individuals.

Each Human Aβ42 US ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

*Please refer to the ELISA protocol for specific reference citations.
For Research Use Only. Not for use in diagnostic procedures.


Gene Aliases: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2
Shipping Condition: Wet Ice
Marker: Aβ 42
Gene ID: 351
Species: Human
Gene Symbol: APP
Sensitivity: <1 pg/mL
Target Gene: amyloid beta (A4) precursor protein
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 1.56-100 pg⁄ml
Sample Volume: 50 µl
Incubation Time: 4 hrs
Detection Method: Colorimetric
Research Category: Neurobiology
Sample Type (Specific): Cerebrospinal Fluid
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols

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