IFN gamma Human ELISA Kit

Catalog number: KHC4022

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human IFN-γ ELISA research use only kit is designed to detect and quantify the level of human IFN-γ in serum and supernatant using 96-well plates and a microplate reader.

Easy to run
Rapid protocol
Convenient precoated, removable 8-well strips

Easy-to-Run Sandwich ELISA
The total assay incubation time is only 2.75 hours. A monoclonal capture antibody specific for Human IFN-γ has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Human IFN-γ content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Human IFN-γ antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human IFN-γ present in the original specimen and the optical density can be read on a standard microplate reader.

Ready-to-Use Human IFN-γ ELISA Kit Contains These Reagents, Buffers, and Supplies:
• Human IFN-γ Antibody Coated 96-Well Plate
• Human IFN-γ Standard
• Standard Diluent Buffer
• Human IFN-γ Detection Antibody
• Streptavidin-HRP
• HRP Diluent
• Wash Buffer Concentrate (25X)
• Stabilized Chromogen, TMB
• Stop Solution
• Plate Covers
• Detailed protocol with validation tests

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want.

Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.

Part of the ELISA Kit Family
We have a wide menu of Life Technologies ELISA research use only kits. With excellent sensitivity, proven specificity, and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about ELISA Kits
Learn more about other Immunoassays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Symbol: IFNG
Assay Incubation Time: 2.75 hr
Assay Range: 15.6-1000 pg/mL
Assay Sensitivity: <4 pg/mL
Concentration: 15.6-1000 pg⁄ml
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Gene Aliases: IFG, IFI
Gene ID: 3458
Gene Name: interferon gamma
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Marker: IFN-γ
Product Line: Novex™
Product Size: 192 assays
Protein Aliases: IFN-gamma, Immune interferon
Protein Name: Interferon gamma
Sample Type (Specific): Cell Culture Supernatants, Serum
Sample Volume: 50 µl
Shipping Condition: Wet Ice
Species: Human
Target (Kit Named): IFN gamma
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01579

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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