Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.
AuthorsOzdemir O,
JournalJ Immunol Methods
PubMed ID17188705
'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.
'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More
Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.
AuthorsKroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L
JournalJ Immunol Methods
PubMed ID1431162
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More
Rapid flow cytometric assay for the assessment of natural killer cell activity.
A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More
Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.
Authors
JournalInt J Cancer
PubMed ID23595559
TNFα regulates intestinal organoids from mice with both defined and conventional microbiota.
Authors
JournalInt J Biol Macromol
PubMed ID32693143
Screening for cell migration inhibitors via automated microscopy reveals a Rho-kinase inhibitor.
Authors
JournalChem Biol
PubMed ID15797222
Proximity to traffic, inflammation, and immune function among women in the Seattle, Washington, area.
Authors
JournalEnviron Health Perspect
PubMed ID19337511
Reduced immunoregulatory CD31+ T cells in patients with atherosclerotic abdominal aortic aneurysm.
Authors
JournalArterioscler Thromb Vasc Biol
PubMed ID16357310
Plasma polychlorinated biphenyl concentrations and immune function in postmenopausal women.
Authors
JournalEnviron Res
PubMed ID24721136
Neutrophils Require Activation to Express Functional Cell-Surface Complement Receptor Immunoglobulin.
Authors
JournalFront Immunol
PubMed ID35317169
Dual blockage of STAT3 and ERK1/2 eliminates radioresistant GBM cells.
Authors
JournalRedox Biol
PubMed ID30986607
Effects of a Chimeric Lysin against Planktonic and Sessile Enterococcus faecalis Hint at Potential Application in Endodontic Therapy.
Authors
JournalViruses
PubMed ID29844267
Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.