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LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

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The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
For Research Use Only. Not for use in diagnostic procedures.


Compatible Cells: Eukaryotic Cells, Mammalian Cells
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Format: Slide(s), Tube(s)
Product Line: LIVE/DEAD®
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols