LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
Citas y referencias (14)
Invitrogen™

LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

El kit de citotoxicidad mediada por células LIVE/DEAD® mide la citotoxicidad mediada por células, activada por linfocina (LAK) y mediadaMás información
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Número de catálogoCantidad
L70101 kit
Número de catálogo L7010
Precio (MXN)
-
Cantidad:
1 kit
El kit de citotoxicidad mediada por células LIVE/DEAD® mide la citotoxicidad mediada por células, activada por linfocina (LAK) y mediada por células T de la sustancia asesina natural (NK). Las células diana se preincuban con la tinción de membrana verde fluorescente DiOC18 y luego se mezclan con células efectoras en presencia de yoduro de propidio, el colorante rojo fluorescente impermeable a la membrana. Las células diana vivas y muertas conservan la tinción de membrana verde-fluorescente; las células diana y efectoras con membranas en riesgo muestran tinción de ácido nucleico rojo-fluorescente; las células efectoras vivas no son fluorescentes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas de mamíferos, células eucariotas
DescripciónKit de citotoxicidad mediada por células LIVE/DEAD™, para células de animales
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubos, portaobjetos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorVerde, rojo
Emission501, 617 nm
Excitation Wavelength Range484, 536 nm
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosLIVE/DEAD
Tipo de productoKit de citotoxicidad mediada por células
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador de 2 °C a 8 °C y proteger de la luz.

Preguntas frecuentes

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

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Citations & References (14)

Citations & References
Abstract
Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.
Authors:Ozdemir O,
Journal:J Immunol Methods
PubMed ID:17188705
'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.
Authors:Bourke S, Mason HS, Borok Z, Kim KJ, Crandall ED, Kemp PJ,
Journal:Respir Res
PubMed ID:15857506
'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More
Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.
Authors:Kroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L
Journal:J Immunol Methods
PubMed ID:1431162
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More
Rapid flow cytometric assay for the assessment of natural killer cell activity.
Authors:Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL
Journal:J Immunol Methods
PubMed ID:8228287
A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More
Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.
Authors:
Journal:Int J Cancer
PubMed ID:23595559
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