The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
For Research Use Only. Not for use in diagnostic procedures.
Mammalian Cells, Eukaryotic Cells
LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
For Use With (Application)
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Cell-Mediated Cytotoxicity Kit
Other Label(s) or Dye(s)
501, 617 nm
Excitation Wavelength Range
484, 536 nm
Contents & Storage
Store in refrigerator at 2°C to 8°C and protect from light.