NuPAGE® Tris-Acetate SDS Running Buffer (20X)

Catalog number: LA0041

Novex™  Related applications: Protein Gel Electrophoresis

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Description

NuPAGE® Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins, in their denatured state, on NuPAGE® Novex® Tris-Acetate gels. NuPAGE® Novex® Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Novex® Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.

Formulation
NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended buffers
Run NuPAGE® Novex® Tris-Acetate gels with NuPAGE® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Novex® Tris-Acetate gels.

Easy-to-use format
Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Type: SDS PAGE Gel
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Buffer Type: Running Buffers
Concentrated: 20 X
Product Size: 500 mL
Gel Compatibility: NuPAGE® Tris-Acetate Gels

Contents & storage

Store at +4°C to 25°C. Guaranteed stable for 6 months unless otherwise specified in the product literature.

Documents

Manuals & protocols

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