Protein Electrophoresis Buffers and Reagents bottle

Finding the right protein gel electrophoresis system is critical to getting consistent and accurate results you need for your research. Reagents and buffers are important components of the protein electrophoresis system. We offer a range of electrophoresis buffers and reagents for gel casting, sample preparation, running, and transferring gels. Pick the protein chemistry below to see the recommended buffers and reagents.

Need help deciding on what system to use? Click to find the right gel for your protein

Recommended buffers & reagents for Bis-tris systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Broad-range MW (6-400 kDa) Bis-tris Denaturing: LDS sample buffer

Reducing: Sample reducing agent
Small to medium-sized proteins (6–260 kDa): MES SDS buffer

Medium to large-size proteins (14–260 kDa): MOPs SDS buffer

Reduced samples: Antioxidant
Bis-tris transfer buffer

Recommended buffers & reagents for Tris-glycine systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Broad-range MW (6-400 kDa) Tris-glycine Denaturing: Tris-glycine SDS sample buffer

Native: Tris-glycine native sample buffer
Denaturing: Tris-glycine SDS buffer

Native: Tris-glycine native buffer
Tris-glycine transfer buffer

Recommended buffers & reagents for Tris-Acetate Systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
High MW
(40-500 kDa)
Tris-acetate Denaturing: LDS sample buffer

Native: Tris-glycine native sample buffer
Denaturing: Tris-acetate SDS buffer

Native: Tris-glycine native buffer
Bis-tris transfer buffer

Recommended buffers & reagents for Tricine systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Low MW
(2.5-40 kDa)
Tricine Denaturing: Tricine SDS sample buffer Tricine SDS buffer Tris-glycine transfer buffer

IEF gel recommended buffers

Zymography gel recommended buffers & reagents

Gel type Sample buffer Running buffer Gel development buffer Gel renaturing buffer
Zymogram gel Tris-glycine SDS sample buffer Tris-glycine SDS running buffer Zymogram development buffer Zymogram renaturing buffer

NativePAGE Bis-Tris gel recommended buffers & reagents

Recommended buffers & reagents for Bis-tris systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Broad-range MW (6-400 kDa) Bis-tris Denaturing: LDS sample buffer

Reducing: Sample reducing agent
Small to medium-sized proteins (6–260 kDa): MES SDS buffer

Medium to large-size proteins (14–260 kDa): MOPs SDS buffer

Reduced samples: Antioxidant
Bis-tris transfer buffer

Recommended buffers & reagents for Tris-glycine systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Broad-range MW (6-400 kDa) Tris-glycine Denaturing: Tris-glycine SDS sample buffer

Native: Tris-glycine native sample buffer
Denaturing: Tris-glycine SDS buffer

Native: Tris-glycine native buffer
Tris-glycine transfer buffer

Recommended buffers & reagents for Tris-Acetate Systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
High MW
(40-500 kDa)
Tris-acetate Denaturing: LDS sample buffer

Native: Tris-glycine native sample buffer
Denaturing: Tris-acetate SDS buffer

Native: Tris-glycine native buffer
Bis-tris transfer buffer

Recommended buffers & reagents for Tricine systems

Protein sample Gel chemistry Sample buffers Running buffer Transfer buffer
Low MW
(2.5-40 kDa)
Tricine Denaturing: Tricine SDS sample buffer Tricine SDS buffer Tris-glycine transfer buffer

IEF gel recommended buffers

Zymography gel recommended buffers & reagents

Gel type Sample buffer Running buffer Gel development buffer Gel renaturing buffer
Zymogram gel Tris-glycine SDS sample buffer Tris-glycine SDS running buffer Zymogram development buffer Zymogram renaturing buffer

NativePAGE Bis-Tris gel recommended buffers & reagents