Tampón de cátodo IEF pH 3-10 (10X)

El tampón de cátodo de IEF de pH 3-10 (10X) es un tampón de cátodo de IEF premezclado y optimizadoMás información

Número de catálogo	Cantidad
LC5310	125 mL

Número de catálogo LC5310

Precio (MXN)

Cantidad:

125 mL

El tampón de cátodo de IEF de pH 3-10 (10X) es un tampón de cátodo de IEF premezclado y optimizado para su uso con geles de IEF Novex™ de pH 3-10. IEF es una técnica sensible que se ve afectada por muchos factores. Los tampones de IEF premezclados y optimizados reducen la variabilidad y garantizan resultados uniformes.

Nota de uso: Las soluciones tampón de cátodo de IEF contienen un reactivo que varía de color blanco a amarillo claro. Esta variación de color puede producir una variación de color similar en la solución final, desde un tono incoloro hasta uno amarillento. Este color no afectará al rendimiento del producto.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

TampónTampones de cátodos y ánodos

Concentración10X

Compatibilidad del gelGeles de IEF Novex™

Tipo de productoTampón de cátodo IEF

Cantidad125 mL

Condiciones de envíoHielo húmedo

Tipo de gelIEF

Intervalo de pHentre 3 y 10

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (2–8° C).

Preguntas frecuentes

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the components of the ZOOM IEF Fractionator?

The ZOOM IEF Fractionator includes the following components:

Chamber Assembly Tube with Anode Reservoir
Spill Trough with Cathode Reservoir Lid
Sample Chambers (7)
Sample Chamber O-ring Seals, red (10)
Sample Chamber Caps with O-rings (7)
Cathode End Sealer
Anode End Sealer
Cathode End Screw Cap
Spacers, black (8)
Spares Box 1
Sample Chamber O-ring Seals (8)
Sample Chamber Caps with O-rings (7)
Spares Box 2
Cathode Chamber Seals (2)
Spacers, black (8)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the storage conditions for the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer?

We recommend storing the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.