NuPAGE Antioxidant prevents sample reoxidation and maintains proteins in a reduced state during protein gel electrophoresis and protein transfer. It is added to the running buffer when performing protein gel electrophoresis under reducing conditions. NuPAGE Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent reoxidation of sensitive amino acids such as methionine and tryptophan.
NuPAGE Antioxidant should be used with samples reduced using NuPAGE Sample Reducing Agent or other reducing agents. NuPAGE Antioxidant is optimized for the neutral pH of NuPAGE gels and is not compatible with other gel chemistries. It may also be added to NuPAGE Transfer Buffer to enhance transfer of proteins to membranes.