Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Bolt LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.
Note: Bolt LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. Bolt LDS Sample Buffer also contains a higher concentration glycerol.
For Research Use Only. Not for use in diagnostic procedures.
Bolt™ Bis-Tris Plus Gels
Sample Loading Buffers
For Use With (Equipment):
Contents & storage
Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.