Bolt Antioxidant prevents sample reoxidation and maintains proteins in a reduced state during protein gel electrophoresis and protein transfer. It is added to the running buffer when performing protein gel electrophoresis under reducing conditions. Bolt Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent reoxidation of sensitive amino acids such as methionine and tryptophan.
Bolt Antioxidant should be used with samples reduced using Bolt Sample Reducing Agent or other reducing agents. It is optimized for the neutral pH of Bolt gels and is not compatible with other gel chemistries. Bolt Antioxidant may also be added to Bolt Transfer Buffer to enhance transfer of proteins to membranes.