Bolt™ Antioxidant

Catalog number:  BT0005

 Related applications:

Protein Assays and Analysis

| Protein Gel Electrophoresis

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Bolt® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. Bolt® Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent re-oxidation of sensitive amino acids such as methionine and tryptophan.

Bolt® Antioxidant must be used with samples reduced using Bolt® Sample Reducing Agent or other reducing agent. Bolt® Antioxidant is optimized for the neutral pH of Bolt® Bis-Tris Plus gels and is not compatible with other gel chemistries.

Bolt® Antioxidant can also be used with Bolt® Transfer Buffer to enhance transfer of reduced proteins to membranes when using the Mini Blot Module.

About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.
For Research Use Only. Not for use in diagnostic procedures.


Buffer Type: Antioxidant
Form: Liquid
Gel Compatibility: Bolt™ Bis-Tris Plus Gels
Product Line: Bolt®
Product Size: 15 ml
Volume (Metric): 15 ml

Contents & storage

Store at 4°C.


Manuals & protocols