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Invitrogen™

NuPAGE™ MES SDS Running Buffer (20X)

Catalog number:  NP0002

 Related applications:

Protein Gel Electrophoresis

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Description

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Formulation: 1X: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3
Gel Type: SDS PAGE Gel
Shipping Condition: Room Temperature
pH: pH 7.3
Buffer Type: Running Buffers
Concentrated: 20 X
Gel Compatibility: NuPAGE® Bis-Tris Gels
Product Line: NuPAGE®
Product Size: 500 mL

Contents & storage

Contents: NuPAGE® MES SDS Running Buffer (20X)
Storage: +4°C to 25°C

Documents

Manuals & protocols