Invitrogen™

NuPAGE™ LDS Sample Buffer (4X)

Catalog number: NP0007
Invitrogen™

NuPAGE™ LDS Sample Buffer (4X)

Catalog number: NP0007
NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol.
 
Catalog Number
NP0007
Unit Size
10 mL
Quantity
10mL
Price (USD)
Price: 21.38
Online price: 20.65
Your price:
Availability
-
Full specifications
Quantity
BufferSample Loading Buffers
Concentration4 X
Gel TypeNuPAGE
Product TypeLDS Sample Buffer
Gel CompatibilityNuPAGE™ Gels
Product LineNuPAGE™
Quantity10mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit Size10 mL
Catalog Number
Unit Size
250 mL
Quantity
250mL
Price (USD)
Price: 169.00
Online price: 161.65
Your price:
Availability
-
Full specifications
Quantity
BufferSample Loading Buffers
Concentration4X
Gel TypeNuPAGE
Product TypeLDS Sample Buffer
Gel CompatibilityNuPAGE™ Gels
Product LineNuPAGE™
Quantity250mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit Size250 mL
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)AvailabilityQuantity
NP0007Full specifications
10 mL10mL
Price: 21.38
Online price: 20.65
Your price:
-
BufferSample Loading Buffers
Concentration4 X
Gel TypeNuPAGE
Product TypeLDS Sample Buffer
Gel CompatibilityNuPAGE™ Gels
Product LineNuPAGE™
Quantity10mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit Size10 mL
NP0008Full specifications
250 mL250mL
Price: 169.00
Online price: 161.65
Your price:
-
BufferSample Loading Buffers
Concentration4X
Gel TypeNuPAGE
Product TypeLDS Sample Buffer
Gel CompatibilityNuPAGE™ Gels
Product LineNuPAGE™
Quantity250mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit Size250 mL
Showing 2 of 2

Use to prepare protein samples for denaturing gel electrophoresis with NuPAGE Novex™ gels. Optimal protein sample preparation for PAGE requires denaturing and reducing protein disulfide bonds.

  • Contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of reducing agent
  • Highly viscous and concentrated solution
  • Contains higher concentration glycerol

NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use.

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.

Store at 4°C to 25°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text