Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Aditivo de muestra NativePAGE™ 5 % G-250
Invitrogen™

Aditivo de muestra NativePAGE™ 5 % G-250

El aditivo de muestras G-250 al 5 % NativePAGE™ se añade a las muestras preparadas con el tampón de muestraMás información
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Número de catálogoCantidad
BN20040,5 mL
Número de catálogo BN2004
Precio (MXN)
-
Cantidad:
0,5 mL
El aditivo de muestras G-250 al 5 % NativePAGE™ se añade a las muestras preparadas con el tampón de muestra NativePAGE™ y detergentes (DMM al 10 % o digitonina al 5 %) justo antes de aplicar las muestras a los geles NativePAGE™. Este aditivo también se incluye en el kit de preparación de muestras NativePAGE™. Las muestras preparadas con un 10 % de DDM y 5 % de digitonina, o el kit de preparación de muestras NativePAGE™, son compatibles con los geles NativePAGE™ Novex Bis-Tris para electroforesis nativa, que muestran un incremento de la resolución y han reducido la presencia de rayas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
TampónTampones de carga de muestras
Compatibilidad del gelGeles NativePAGE™
Línea de productosNativePAGE
Tipo de productoAditivo de muestras PAGE G-250
Cantidad0,5 mL
Duración de almacenamiento1 año
Condiciones de envíoTemperatura ambiente
Tipo de gelGel Native
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente

Preguntas frecuentes

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the purpose of using the NativePAGE 5% G-250 Sample Additive?

The NativePAGE 5% G-250 Sample Additive is a concentrated stock solution of Coomassie G-250 designed for use with detergent (non-ionic) containing samples prepared for NativePAGE gel electrophoresis. Native proteins normally migrate on the gel according to natural charge/pI in the pH of gel buffer, but adding the Coomassie G-250 renders an associated negative charge even to high pI proteins that would normally have a positive charge. It binds to proteins non-specifically without denaturing them. The NativePAGE 5% G-250 Sample Additive is added to the cathode buffer and, optionally, to samples as well.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the composition of the NativePAGE 5% G-250 Sample Additive? Does it contain a detergent?

The NativePAGE 5% G-250 Sample Additive (Cat. No. BN2004) is a concentrated stock solution of Coomassie G-250 designed for use with detergent (non-ionic) containing samples prepared for NativePAGE gel electrophoresis. The exact composition of the NativePAGE 5% G-250 Sample Additive is proprietary. It does not contain a detergent.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Aditivo de muestra NativePAGE™ 5 % G-250 FAQs