Invitrogen™

Novex™ Tricine SDS Sample Buffer (2X)

Catalog number:  LC1676

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. Novex Tricine SDS Sample Buffer is specifically formulated for optimal electrophoresis of small proteins and peptides. The sample buffer is formulated with Coomassie Blue G and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie Blue G gives a sharp dye front with Tricine SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.

Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.

See all buffers and reagents for SDS-PAGE ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Name or Material: Sample Loading Buffers
Concentration: 2 X
Product Line: Novex™
Gel Compatibility: Novex™ Tricine Gels
Gel Type: SDS PAGE Gel
Shipping Condition: Wet Ice

Contents & storage

Tricine Sample Buffer (2X) containing sodium dodecyl sulfate (SDS) at pH 8.45 with Coomassie Blue G and phenol red.

Store at 2°C to 8°C.