NuPAGE Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins in their denatured state on Tris-Acetate gels. NuPAGE Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer. NuPAGE Tris-Acetate gels can also be run with Novex Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.
Recommended buffers Run NuPAGE Tris-Acetate gels with NuPAGE Tris-Acetate SDS Running Buffer and to ensure good sample reduction and band resolution, use NuPAGE LDS Sample Buffer. For native running conditions, the Tris-Glycine Native running and sample buffers should be used.