Invitrogen™

MagicMark™ XP Western Protein Standard

Catalog number:  LC5602

 Related applications:

Protein Electrophoresis & Western Blotting

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Applications
• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: User defined detection system
Molecular Weight: 220, 120, 100, 80, 60, 50, 40, 30, 20 kDa
Number of Markers: 9
Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex® Tricine Gels, Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, SDS-PAGE Gels
Stain Type: Unstained
Size Range: 20 to 220 kDa
Product Line: MagicMark™
Product Size: 250 µL
System: Western Blotting, SDS-PAGE
Shipping Condition: Dry Ice

Contents & storage

250 µl of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue. Store at -20°C.

Documents

Manuals & protocols