Invitrogen™
BenchMark™ Fluorescent Protein Standard
Description
BenchMark Fluorescent Protein Standard consists of a wide molecular weight range of proteins (~11–155 kDa) conjugated to the fluorescent dye Alexa Fluor 488, allowing direct visualization using fluorescence. The proteins can be resolved into sharp bands by SDS-PAGE and visualized using a UV transilluminator, CCD camera with appropriate filters, or laser scanner systems.Compare and view all other protein standards and ladders ›
Product features
• Visualization: fluorescence at 488 nm channel, maximum excitation at 493 nm and maximum emission at 516 nm
• Ready-to-use format: The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use
Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on fluorescent SDS-polyacrylamide gels and western blots
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Detection Method: | 488nm channel |
|---|---|
| Gel Compatibility: | Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels |
| Number of Markers: | 7 |
| Product Line: | BenchMark™ |
| Shipping Condition: | Dry Ice |
| Size Range: | 11 to 155 kDa |
| System Type: | SDS-PAGE |
| Molecular Weight: | 155, 98, 63, 40, 32, 21, 11 kDa |
| Stain Type: | bands conjugated to Alexa Fluor 488 |
Contents & storage
125 µl (25-50 applications) is provided in loading buffer consisting of Tris-HCl, SDS, Glycerol, and Coomassie® Blue G-250. Store at -20°C. Avoid repeated freezing and thawing. Stable for 6 months when properly stored.
