The Lumio™ Green Detection Kit is a sensitive and highly specific kit for labeling Lumio™ fusion proteins prior to electrophoresis. The Lumio™ Green Kit enables immediate visualization of Lumio™ fusion protein bands directly in a polyacrylamide gel. Using the Lumio™ Green Detection Kit you'll:
• Label proteins fused to a Lumio™ sequence • Visualize results immediately after electrophoresis—eliminate gel staining and western blotting • Detect nanogram levels of protein with a UV transilluminator, equipped with a standard camera (Figure 1), or a laser-based scanner The Lumio™ Green Detection Reagent, included in the Lumio™ Green Detection Kit, can also be used for real-time detection of protein synthesis during in vitro transcription-translation reactions.
Immediate and Effortless Detection To detect using the Lumio™ Green Detection Kit simply: 1. Add the Lumio™ Green Detection Reagent and the optimized Lumio™ Sample Buffer to your protein sample and heat for 10 minutes at 70°C. 2. Add the Lumio™ In-Gel Detection Enhancer to your sample mix and incubate for 5 minutes at room temperature. 3. Load and electrophorese your protein gel. 4. Visualize your Lumio™ fusion protein bands with a UV transilluminator, equipped with a standard camera, or laser-based scanner. 5. After documenting your Lumio™ Green signal, total protein staining can be performed.
For Research Use Only. Not for use in diagnostic procedures.
Label or Dye
Pre-Gel Fusion Protein Labeling Kit
Proteins (Lumio™-Tagged) , Proteins (TC-Tagged)
Contents & Storage
The Lumio™ Green Detection Kit contains one vial of Lumio™ Green Detection Reagent, five vials of Lumio™ Gel Sample Buffer, and one vial of Lumio™ In-Gel Detection Enhancer. Store at -20°C. All components are guaranteed stable for 6 months when properly stored.