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Invitrogen

IL-10 Monoclonal Antibody (12G8), Biotin

18 References
View all (112) IL-10 antibodies

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Product Details

M011B

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1000
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
View 17 publications 17 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

12G8

Immunogen

Recombinant human IL-10 (COS cell-derived)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

250 µg

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223553

Product Specific Information

M011B targets IL-10 in ELISA and Western blot applications and shows reactivity with human samples.



The M011B immunogen is recombinant human IL-10 (COS cell-derived). The original parent clone is 12G8 and has been subcloned as 2553.2.4.



M011B detects IL-10 which has a predicted molecular weight of approximately 19 kDa.



The M011B IL10 antibody (biotinylated conjugate of clone 12G8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M010 (clone 9D7). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Biotinylated antibody M011B (clone 12G8) and antibody M010 (clone 9D7) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.



This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cytokine synthesis inhibitory factor; H-IL-10; il 10; IL10; ILN; Interleukin; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)
cytokine activity interleukin-10 receptor binding protein binding growth factor activity protein dimerization activity
Process(es)
negative regulation of cytokine production positive regulation of cytokine production positive regulation of endothelial cell proliferation response to molecule of bacterial origin positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response positive regulation of B cell apoptotic process immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus regulation of gene expression negative regulation of autophagy response to activity response to inactivity cytokine-mediated signaling pathway hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of B cell proliferation response to lipopolysaccharide negative regulation of interferon-alpha production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production response to insulin negative regulation of heterotypic cell-cell adhesion positive regulation of heterotypic cell-cell adhesion response to carbon monoxide type 2 immune response B cell proliferation negative regulation of T cell proliferation positive regulation of tyrosine phosphorylation of STAT protein positive regulation of macrophage activation negative regulation of apoptotic process negative regulation of neuron apoptotic process negative regulation of nitric oxide biosynthetic process T-helper 1 cell differentiation regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of cell cycle positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade negative regulation of inflammatory response regulation of synapse organization regulation of B cell activation negative regulation of membrane protein ectodomain proteolysis response to glucocorticoid negative regulation of cytokine activity cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production endothelial cell apoptotic process liver regeneration interleukin-10-mediated signaling pathway positive regulation of plasma cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of response to wounding negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of complement-dependent cytotoxicity positive regulation of sprouting angiogenesis negative regulation of vascular smooth muscle cell proliferation positive regulation of vascular smooth muscle cell proliferation negative regulation of endothelial cell apoptotic process
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