Mitochondrial modulation of Ca2+ -induced Ca2+ -release in rat sensory neurons.
AuthorsJackson JG, Thayer SA
JournalJ Neurophysiol
PubMed ID16760347
'Ca2+ -induced Ca2+ -release (CICR) from ryanodine-sensitive Ca2+ stores provides a mechanism to amplify and propagate a transient increase in intracellular calcium concentration ([Ca2+]i). A subset of rat dorsal root ganglion neurons in culture exhibited regenerative CICR when sensitized by caffeine. [Ca2+]i oscillated in the maintained presence of 5 mM ... More
Rapid functional assays of recombinant IP3 receptors.
AuthorsLaude AJ, Tovey SC, Dedos SG, Potter BV, Lummis SC, Taylor CW
JournalCell Calcium
PubMed ID15963563
'Functional assays of inositol 1,4,5-trisphosphate receptors (IP3R) currently use 45Ca2+ release methods, fluorescent Ca2+ indicators within either the ER or cytosol, or electrophysiological analyses of IP3R in the nuclear envelope or artificial bilayers. None of the methods is presently amenable to the rapid, high-throughput quantitative analyses of IP3R function needed ... More
The endoplasmic reticulum: one continuous or several separate Ca(2+) stores?
AuthorsPetersen OH, Tepikin A, Park MK
JournalTrends Neurosci
PubMed ID11311379
The Ca2+ store and sink in the endoplasmic reticulum (ER) is important for Ca2+ signal integration and for conveyance of information in spatial and temporal domains. Textbooks regard the ER as one continuous network, but biochemical and biophysical studies revealed apparently discrete ER Ca2+ stores. Recent direct studies of ER ... More
Real-time observations of intracellular Mg2+ signaling and waves in a single living ventricular myocyte cell.
AuthorsLee S, Lee HG, Kang SH,
JournalAnal Chem
PubMed ID19086893
Despite the important regulatory role of Mg(2+) in metabolic pathways, its underlying mechanism is not completely understood at the single-cell level. This study examined the propagation and dynamics of Mg(2+) signaling across the cell membrane by employing the real-time visualization of intracellular Mg(2+) waves in living ventricular myocytes using a ... More
TRPC channels determine human keratinocyte differentiation: new insight into basal cell carcinoma.
AuthorsBeck B, Lehen'kyi V, Roudbaraki M, Flourakis M, Charveron M, Bordat P, Polakowska R, Prevarskaya N, Skryma R,
JournalCell Calcium
PubMed ID17920677
Aberrant keratinocyte differentiation is considered to be a key mechanism in the onset of hyperproliferative dermatological diseases, including basal cell carcinoma (BCC). It is, therefore, vital to understand what drives keratinocytes to develop such pathological phenotypes. The role of calcium in keratinocyte differentiation is uncontested but the mechanisms controlling calcium-induced ... More
Role of glycolytically generated ATP for CaMKII-mediated regulation of intracellular Ca2+ signaling in bovine vascular endothelial cells.
AuthorsAromolaran AS, Zima AV, Blatter LA,
JournalAm J Physiol Cell Physiol
PubMed ID17344311
The role of glycolytically generated ATP in Ca(2+)/calmodulin-dependent kinase II (CaMKII)-mediated regulation of intracellular Ca(2+) signaling was examined in cultured calf pulmonary artery endothelial (CPAE) cells. Exposure of cells (extracellular Ca(2+) concentration = 2 mM) to glycolytic inhibitors 2-deoxy-D-glucose (2-DG), pyruvate (pyr) + beta-hydroxybutyrate (beta-HB), or iodoacetic acid (IAA) caused ... More
Relocalization of STIM1 for activation of store-operated Ca(2+) entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca(2+) store.
AuthorsOng HL, Liu X, Tsaneva-Atanasova K, Singh BB, Bandyopadhyay BC, Swaim WD, Russell JT, Hegde RS, Sherman A, Ambudkar IS,
JournalJ Biol Chem
PubMed ID17298947
STIM1 (stromal interacting molecule 1), an endoplasmic reticulum (ER) protein that controls store-operated Ca(2+) entry (SOCE), redistributes into punctae at the cell periphery after store depletion. This redistribution is suggested to have a causal role in activation of SOCE. However, whether peripheral STIM1 punctae that are involved in regulation of ... More
The anti-apoptotic protein Mcl-1 inhibits mitochondrial Ca2+ signals.
AuthorsMinagawa N, Kruglov EA, Dranoff JA, Robert ME, Gores GJ, Nathanson MH
JournalJ Biol Chem
PubMed ID16027162
Apoptosis contributes to the regulation of cell growth and regeneration and to the development of neoplasia. Mcl-1 is an anti-apoptotic protein that is particularly important for the development of hematological and biliary malignancies, but the mechanism of action of Mcl-1 is unknown. A number of pro- and anti-apoptotic proteins exhibit ... More
The endoplasmic reticulum as one continuous Ca(2+) pool: visualization of rapid Ca(2+) movements and equilibration.
AuthorsPark MK, Petersen OH, Tepikin AV
JournalEMBO J
PubMed ID11060024
We investigated whether the endoplasmic reticulum (ER) is a functionally connected Ca(2+) store or is composed of separate subunits by monitoring movements of Ca(2+) and small fluorescent probes in the ER lumen of pancreatic acinar cells, using confocal microscopy, local bleaching and uncaging. We observed rapid movements and equilibration of ... More
Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum.
AuthorsEchevarría W, Leite MF, Guerra MT, Zipfel WR, Nathanson MH
JournalNat Cell Biol
PubMed ID12717445
Calcium is a second messenger in virtually all cells and tissues. Calcium signals in the nucleus have effects on gene transcription and cell growth that are distinct from those of cytosolic calcium signals; however, it is unknown how nuclear calcium signals are regulated. Here we identify a reticular network of ... More
Correlations between the functional integrity of the endoplasmic reticulum and polarized Ca2+ signalling in mouse lacrimal acinar cells: a role for inositol 1,3,4,5-tetrakisphosphate.
AuthorsHarmer AR, Gallacher DV, Smith PM
JournalBiochem J
PubMed ID12117415
Ca(2+) signalling in exocrine acinar cells has been shown to be both polarized and pulsatile in all cell types tested, except acutely isolated mouse lacrimal acinar cells. Lacrimal cells are also unusual in that they display a very low sensitivity to Ins(1,4,5) P (3) (Ins P (3)) that may be ... More
Phosphorylation of SHP-2 regulates interactions between the endoplasmic reticulum and focal adhesions to restrict interleukin-1-induced Ca2+ signaling.
AuthorsWang Q, Herrera Abreu MT, Siminovitch K, Downey GP, McCulloch CA
JournalJ Biol Chem
PubMed ID16905534
Interleukin-1 (IL-1)-induced Ca2+ signaling in fibroblasts is constrained by focal adhesions. This process involves the proteintyrosine phosphatase SHP-2, which is critical for IL-1-induced phosphorylation of phospholipase Cgamma1, thereby enhancing IL-1-induced Ca2+ release and ERK activation. Currently, the mechanisms by which SHP-2 modulates Ca2+ release from the endoplasmic reticulum are not ... More
A calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes.
AuthorsLi N, Sul JY, Haydon PG
JournalJ Neurosci
PubMed ID14614089
The roles of nitric oxide are primarily undefined in astrocytes, cells that are active partners in synaptic transmission. Because nitric oxide synthases are present in astrocytes, we imaged the formation of nitric oxide in cultured murine cortical astrocytes using DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate). We demonstrated that physiological concentrations of ATP induced ... More
Dependence of transient and residual calcium dynamics on action-potential patterning during neuropeptide secretion.
AuthorsMuschol M, Salzberg BM
JournalJ Neurosci
PubMed ID10995820
Secretion of the neuropeptide arginine vasopressin (AVP) from the neurohypophysis is optimized by short phasic bursts of action potentials with a mean intraburst frequency around 10 Hz. Several hypotheses, most prominently action-potential broadening and buildup of residual calcium, have been proposed to explain this frequency dependence of AVP release. However, ... More
BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites.
Authors
JournalCell Rep
PubMed ID33691099
Optical Recording of Action Potential Initiation and Propagation in Mouse Skeletal Muscle Fibers.
Authors
JournalBiophys J
PubMed ID30448039
Impaired calcium homeostasis via advanced glycation end products promotes apoptosis through endoplasmic reticulum stress in human nucleus pulposus cells and exacerbates intervertebral disc degeneration in rats.
Authors
JournalFEBS J
PubMed ID31230413
iRhom pseudoproteases regulate ER stress-induced cell death through IP3 receptors and BCL-2.
Authors
JournalNat Commun
PubMed ID35273168
Cutting Edge: Imbalanced Cation Homeostasis in MAGT1-Deficient B Cells Dysregulates B Cell Development and Signaling in Mice.
Authors
JournalJ Immunol
PubMed ID29581357
A Temporal Examination of Cytoplasmic Ca2 + Levels, Sarcoplasmic Reticulum Ca2 + Levels, and Ca2 + -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels.
Authors
JournalFront Physiol
PubMed ID33192559
A miniature Couette to generate shear for flow cytometry: studying real-time modulation of intracellular calcium in monocytic cells.
Authors
JournalCytometry A
PubMed ID22045643
3,3'-Diindolylmethane induces gastric cancer cells death via STIM1 mediated store-operated calcium entry.
Authors
JournalInt J Biol Sci
PubMed ID33867841
Probing novel roles of the mitochondrial uniporter in ovarian cancer cells using nanoparticles.
Authors
JournalJ Biol Chem
PubMed ID23615904
Cytosolic calcium coordinates mitochondrial energy metabolism with presynaptic activity.