MitoProbe™ Transition Pore Assay Kit, for flow cytometry

Catalog number: M34153

Molecular Probes™  Related applications: Cell Structure | Cell Viability, Proliferation & Function | Flow Cytometry

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Description

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: calcein AM
Sub-Cellular Localization: Mitochondria
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 100
For Use With (Equipment): Flow Cytometer
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 494⁄517
Shipping Condition: Room Temperature

Contents & storage

Contains 5 vials of calcein, AM (50 µg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 µg), calcium salt, and 1 vial of DMSO (1.5 mL).

Store in freezer (-5 to -30°C).

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