Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.
AuthorsNguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E,
JournalJ Biol Chem
PubMed ID21930693
The mitochondrial permeability transition pore (mPTP) opening plays a critical role in mediating cell death during ischemia/reperfusion (I/R) injury. Our previous studies have shown that cysteine 203 of cyclophilin D (CypD), a critical mPTP mediator, undergoes protein S-nitrosylation (SNO). To investigate the role of cysteine 203 in mPTP activation, we ... More
Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.
AuthorsPetronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F
JournalBiophys J
PubMed ID9929477
'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More
Calpain and caspase orchestrated death signal to accomplish apoptosis induced by resveratrol and its novel analog hydroxystilbene-1 [correction of hydroxstilbene-1] in cancer cells.
AuthorsGuha P, Dey A, Dhyani MV, Sen R, Chatterjee M, Chattopadhyay S, Bandyopadhyay SK,
JournalJ Pharmacol Exp Ther
PubMed ID20484155
'Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy ... More
Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts.
AuthorsCotán D, Cordero MD, Garrido-Maraver J, Oropesa-Ávila M, Rodríguez-Hernández A, Gómez Izquierdo L, De la Mata M, De Miguel M, Lorite JB, Infante ER, Jackson S, Navas P, Sánchez-Alcázar JA,
JournalFASEB J
PubMed ID21551238
Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease most usually caused by point mutations in tRNA genes encoded by mtDNA. Here, we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from 2 patients with MELAS who harbored the A3243G mutation. Both ... More
Imaging the mitochondrial permeability transition pore in intact cells.
AuthorsPetronilli V, Miotto G, Canton M, Colonna R, Bernardi P, Di Lisa F
JournalBiofactors
PubMed ID9914828
The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of ... More
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis.
'Peroxynitrite is a highly reactive nitrogen species and a potent inducer of apoptosis and necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as a major cause of impaired sperm function; however, its ability to trigger cell death has not been described in human spermatozoa. The objective here was to ... More
Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS-mediated mitochondrial dysfunction.
AuthorsYao H, Yuan Z, Wei G, Chen C, Duan J, Li Y, Wang Y, Zhang C, Liu Y
JournalMol Med Rep
PubMed ID28990067
'Cerebral ischemia and following reperfusion affects many people worldwide. To discover efficient therapeutic approaches, numerous natural products have been investigated. The current study investigated the protective effects of thevetiaflavone, a natural flavonoid obtained from Wikstroemia indica, and the associated mechanisms using PC12 cells induced by oxygen and glucose deprivation. As a ... More
Pharmacological protection of mitochondrial function mitigates acute limb ischemia/reperfusion injury.
AuthorsBi W, Bi Y, Gao X, Yan X, Zhang Y, Harris J, Legalley TD, Gibson KM, Bi L
JournalBioorg Med Chem Lett
PubMed ID27390069
'We describe several novel curcumin analogues that possess both anti-inflammatory antioxidant properties and thrombolytic activities. The therapeutic efficacy of these curcumin analogues was verified in a mouse ear edema model, a rat arterial thrombosis assay, a free radical scavenging assay performed in PC12 cells, and in both in vitro and ... More
Effect of different metals on oxidative state and mitochondrial membrane potential in trout erythrocytes.
AuthorsSilvestri S, Orlando P, Brugè F, Falcioni G, Tiano L
JournalEcotoxicol Environ Saf
PubMed ID27566895
Homeostasis of metal ions is critical for life and excessive exposure can promote cellular damage that could be due to oxidative damage. In this context we evaluated the effects of three different elements (copper, zinc and aluminum) on oxidative stress and mitochondrial functionality in nucleated trout erythrocytes (Oncorhynchus mykiss). Flowcytometric ... More
Resveratrol protects PC12 cells against OGD/ R-induced apoptosis via the mitochondrial-mediated signaling pathway.
AuthorsLiu X, Zhu X, Chen M, Ge Q, Shen Y, Pan S
JournalActa Biochim Biophys Sin (Shanghai)
PubMed ID26960953
In this study, we investigated the neuroprotective potential of resveratrol against oxygen glucose deprivation/reoxygenation (OGD/R)-induced apoptotic damages in well-differentiated PC12 cells and the underlying mechanisms. Cells were incubated under normal condition or OGD/R in the presence or absence of 10 µM resveratrol. Cell viability was determined with methyl-thiazolyl-tetrazolium (MTT) assay. ... More
A small-molecule inhibitor of TRPC5 ion channels suppresses progressive kidney disease in animal models.
AuthorsZhou Y, Castonguay P, Sidhom EH, Clark AR, Dvela-Levitt M, Kim S, Sieber J, Wieder N, Jung JY, Andreeva S, Reichardt J, Dubois F, Hoffmann SC, Basgen JM, Montesinos MS, Weins A, Johnson AC, Lander ES, Garrett MR, Hopkins CR, Greka A
JournalScience
PubMed ID29217578
Progressive kidney diseases are often associated with scarring of the kidney's filtration unit, a condition called focal segmental glomerulosclerosis (FSGS). This scarring is due to loss of podocytes, cells critical for glomerular filtration, and leads to proteinuria and kidney failure. Inherited forms of FSGS are caused by Rac1-activating mutations, and ... More
Indole-TEMPO conjugates alleviate ischemia-reperfusion injury via attenuation of oxidative stress and preservation of mitochondrial function.
AuthorsBi W, Bi Y, Gao X, Li P, Hou S, Zhang Y, Bammert C, Jockusch S, Legalley TD, Michael Gibson K, Bi L
JournalBioorg Med Chem
PubMed ID28359673
Mitochondrial oxidative damage contributes to a wide range of pathologies including ischemia/reperfusion injury. Accordingly, protecting mitochondria from oxidative damage should possess therapeutic relevance. In the present study, we have designed and synthesized a series of novel indole-TEMPO conjugates that manifested good anti-inflammatory properties in a murine model of xylene-induced ear ... More
Cryopreservation induces mitochondrial permeability transition in a bovine sperm model.
AuthorsTreulen F, Arias ME, Aguila L, Uribe P, Felmer R
JournalCryobiology
PubMed ID29864412
When the mitochondria of somatic cells are exposed to pathological calcium overload, these trigger mitochondrial permeability transition (MPT) leading to mitochondrial dysfunction and cell death. Cryopreservation procedures expose mammalian spermatozoa to physical and chemical stressors, which affect plasma membrane integrity and induce a pathological calcium overload that gradually promotes loss ... More
AuthorsHuang Y, Ye Z, Ma T, Li H, Zhao Y, Chen W, Wang Y, Yan X, Gao Y, Li Z
JournalExp Eye Res
PubMed ID29407220
Mitochondrial components are of great importance for the maintenance of lens transparency. In our previous work, we confirmed that carbon monoxide (CO) can protect human and rabbit lens epithelial cells (LECs) from hydrogen peroxide (H ... More
Activation of AMPK inhibits inflammatory response during hypoxia and reoxygenation through modulating JNK-mediated NF-?B pathway.
AuthorsChen X, Li X, Zhang W, He J, Xu B, Lei B, Wang Z, Cates C, Rousselle T, Li J,
JournalMetabolism
PubMed ID29526538
AMP-activated Protein Kinase (AMPK) is a stress-activated kinase that protects against cardiomyocyte injury during ischemia and reperfusion. c-Jun N-terminal kinase (JNK), a mitogen activated protein kinase, is activated by ischemia and reperfusion. NF-?B is an important transcription factor involved in ischemia and reperfusion injury. The intrinsic activation of AMPK attenuates ... More
Effects of elamipretide on skeletal muscle in dogs with experimentally induced heart failure.
Elamipretide (ELAM), an aromatic-cationic tetrapeptide, interacts with cardiolipin and normalizes dysfunctional mitochondria of cardiomyocytes. This study examined the effects of ELAM on skeletal muscle mitochondria function in dogs with chronic heart failure (HF). Studies were performed in skeletal muscle biopsy specimens obtained from normal dogs (n = 7) and dogs with chronic ... More