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Invitrogen
RAP1A Monoclonal Antibody (1D2-1C64)
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FIGURE: 1 / 5
RAP1A Antibody (MA1-013) in ICC/IF
Immunofluorescent analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody (Product # MA1-013) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conj... View More
Product Details
MA1-013
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1D2-1C64
Immunogen
Recombinant full-length human Rap1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Western blot analysis of MA1-013 detects an ~21kDa protein.
Target Information
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein SMG-P21A; Ras-related protein Krev-1; Ras-related protein Rap-1A; small GTP binding protein; unnamed protein product
Gene Aliases: AI848598; C21KG; G-22K; KREV-1; KREV1; RAP1; RAP1A; SMGP21
UniProt ID: (Human) P62834, (Mouse) P62835
Entrez Gene ID: (Human) 5906, (Mouse) 109905
nucleotide binding
GTPase activity
obsolete small monomeric GTPase activity
guanyl-nucleotide exchange factor activity
protein binding
GTP binding
hydrolase activity
GDP binding
small GTPase binding
macromolecular complex binding
protein transporter activity
Ras GTPase binding
Rap guanyl-nucleotide exchange factor activity
protein complex binding
small GTPase
signal transduction
small GTPase mediated signal transduction
nervous system development
response to carbohydrate
positive regulation of neuron projection development
synaptic vesicle exocytosis
Rap protein signal transduction
negative regulation of collagen biosynthetic process
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
positive regulation of glucose import
positive regulation of phagocytosis
positive regulation of Fc receptor mediated stimulatory signaling pathway
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
protein localization to plasma membrane
response to antineoplastic agent
liver regeneration
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
regulation of cell junction assembly
cellular response to forskolin
cellular response to nerve growth factor stimulus
negative regulation of synaptic vesicle exocytosis
positive regulation of vasculogenesis
protein transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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