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Invitrogen
GRK1 Monoclonal Antibody (G8)
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FIGURE: 1 / 21
GRK1 Antibody (MA1-720) in ICC/IF
Immunofluorescence analysis of Rhodopsin Kinase 1a was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rhodopsin Kinase 1a (G8) Mouse Monoclonal Antibody (Product # MA1-720) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
MA1-720
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Human,
Mouse,
Rat
Published species
Bovine,
Dog,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
G8
Immunogen
Full length human GRK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-720 detects G-protein-associated rhodopsin kinase 1a (GRK1a) from human, mouse, rat, bovine and chicken tissues.
MA1-720 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with MA1-720 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.
The MA1-720 immunogen is full length human GRK1. This antibody recognizes an epitope in the C-terminal region of GRK1a.
Target Information
GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK; unnamed protein product
Gene Aliases: GPRK1; GRK1; RHOK; RK
UniProt ID: (Human) Q15835, (Bovine) P28327, (Rat) Q63651
Entrez Gene ID: (Chicken) 395431, (Human) 6011, (Bovine) 281457, (Mouse) 24013, (Rat) 81760
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
G-protein coupled receptor kinase activity
ATP binding
kinase activity
transferase activity
rhodopsin kinase activity
protein binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
signal transduction
visual perception
regulation of G-protein coupled receptor protein signaling pathway
response to light stimulus
regulation of signal transduction
rhodopsin mediated signaling pathway
regulation of rhodopsin mediated signaling pathway
protein autophosphorylation
protein phosphorylation
photoreceptor cell morphogenesis
phosphorylation
termination of G-protein coupled receptor signaling pathway
positive regulation of phosphorylation
negative regulation of apoptotic process
response to stimulus
post-embryonic retina morphogenesis in camera-type eye
response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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