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LIN28A Monoclonal Antibody (14E6-4E6), DyLight™ 650
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FIGURE: 1 / 3
LIN28A Antibody (MA1-016-D650) in ICC/IF
Immunofluorescent analysis of Lin28 (red) showing cytoplasmic staining of NCCIT cells (right panel) compared to HeLa cells (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated Lin28 monoclonal antibody (Product # MA1-016-D650) in 3% BSA-PBS at a dilution of 1:100 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Ima... View More
Product Details
MA1-016-D650
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
14E6-4E6
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-016-D650 has been successfully used in ICC/IF and flow cytometry applications on human and mouse samples.
MA1-016-D650 can be used for immunofluorescence analysis of LIN28 in human iPSCs.
Target Information
Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: FLJ12457; Lin-28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; unnamed protein product; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
Gene Aliases: AL024421; CSDD1; ENSMUSG00000070700; Gm10299; LIN-28; lin-28A; LIN28; LIN28A; Tex17; ZCCHC1
UniProt ID: (Human) Q9H9Z2, (Mouse) Q8K3Y3
Entrez Gene ID: (Human) 79727, (Mouse) 83557
germ cell development
miRNA metabolic process
miRNA catabolic process
posttranscriptional regulation of gene expression
negative regulation of translation
stem cell population maintenance
gene silencing by RNA
pre-miRNA processing
RNA 3'-end processing
positive regulation of TOR signaling
positive regulation of neuron differentiation
negative regulation of glial cell differentiation
positive regulation of translation
stem cell differentiation
positive regulation of protein kinase B signaling
regulation of gene silencing by miRNA
cellular response to glucose stimulus
positive regulation of cell proliferation involved in kidney development
negative regulation of pre-miRNA processing
positive regulation of cytoplasmic translation
regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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