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Invitrogen
Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 550
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FIGURE: 1 / 3
Nanog Antibody (MA1-017-D550) in ICC/IF
Immunofluorescent analysis of Nanog (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated Nanog monoclonal antibody (Product # MA1-017-D550) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with... View More
Product Details
MA1-017-D550
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
23D2-3C6
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-017-D550 has been successfully used in ICC/IF applications with human samples.
MA1-017-D550 can be used for immunofluoresence analysis of Nanog in human iPSCs.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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