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Invitrogen
PSD-95 Monoclonal Antibody (7E3-1B8)
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FIGURE: 1 / 12
PSD-95 Antibody (MA1-046) in ICC/IF
Immunofluorescent analysis of PSD95 using Anti-PSD95 Monoclonal Antibody (7E3-1B8) (Product # MA1-046) shows staining in Hela Cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 (Product # MA1-046) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 3... View More
Product Details
MA1-046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Xenopus
Published species
Avian,
Bovine,
Chicken,
Human,
Invertebrate,
Marsupial,
Mouse,
Non-human primate,
Rabbit,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
7E3-1B8
Immunogen
Purified recombinant rat PSD-95.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-046 detects post synaptic density 95 (PSD-95) from human, rat, xenopus laevis and mouse tissues.
MA1-046 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects an ~95 kDa protein representing PSD-95 from rat brain extracts. Immunofluorescence staining of PSD-95 in rat hippocampal cells with MA1-046 yields a staining pattern coincident with NMDA receptor.
In immunofluorescence staining, fixation with cold methanol is recommended.
The MA1-046 immunogen is purified recombinant rat PSD-95.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Target Information
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: discs large homolog 4; Disks large homolog 4; FLJ97752; FLJ98574; post synaptic density; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; SAP-90; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15; unnamed protein product
Gene Aliases: DLG4; Dlgh4; MRD62; PSD-95; PSD95; SAP-90; SAP90; SAP90A
UniProt ID: (Human) P78352, (Rat) P31016, (Mouse) Q62108
Entrez Gene ID: (Human) 1742, (Rat) 29495, (Mouse) 13385, (Western clawed frog) 779559
protein binding
kinase binding
protein kinase binding
protein phosphatase binding
PDZ domain binding
protein binding, bridging
beta-1 adrenergic receptor binding
D1 dopamine receptor binding
P2Y1 nucleotide receptor binding
acetylcholine receptor binding
ionotropic glutamate receptor binding
macromolecular complex binding
neuroligin family protein binding
scaffold protein binding
guanylate kinase activity
receptor binding
protein C-terminus binding
protein complex binding
glutamate receptor binding
structural molecule activity
neurexin family protein binding
scaffold/adaptor protein
negative regulation of receptor internalization
signal transduction
positive regulation of cytosolic calcium ion concentration
chemical synaptic transmission
nervous system development
learning
synaptic vesicle maturation
social behavior
protein localization to synapse
locomotory exploration behavior
cellular response to potassium ion
establishment of protein localization
establishment or maintenance of epithelial cell apical/basal polarity
regulation of long-term neuronal synaptic plasticity
positive regulation of synaptic transmission
neuromuscular process controlling balance
dendritic spine morphogenesis
protein-containing complex assembly
vocalization behavior
AMPA glutamate receptor clustering
receptor localization to synapse
cell-cell adhesion
NMDA selective glutamate receptor signaling pathway
regulation of postsynaptic membrane neurotransmitter receptor levels
neurotransmitter receptor localization to postsynaptic specialization membrane
positive regulation of neuron projection arborization
positive regulation of excitatory postsynaptic potential
regulation of grooming behavior
protein complex assembly
neuronal ion channel clustering
nucleotide phosphorylation
regulation of N-methyl-D-aspartate selective glutamate receptor activity
locomotory behavior
response to cocaine
regulation of neuronal synaptic plasticity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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