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FIGURE: 1 / 6
SHIP1 Antibody (MA1-10450) in ICC/IF
Immunofluorescence analysis of SHIP1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SHIP1 Mouse Monoclonal Antibody (SHIP-02) (Product # MA1-10450) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
MA1-10450
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
SHIP-02
Immunogen
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Western Blot: Reducing conditions.
Target Information
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeliod cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hp51CN; Inositol polyphosphate-5-phosphatase D; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145 kDa; inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; MGC104855; MGC142140; MGC142142; OTTHUMP00000165069; OTTHUMP00000165070; OTTHUMP00000165071; OTTHUMP00000165072; OTTHUMP00000203442; p150Ship; phosphatidylin; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; Phosphatidylinositol-4,5-bisphosphate 5-phosphatase; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2-containing inositol phosphatase SHIP; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II; SIP-145; Src homology 2 domain-containing inositol-5-phosphatase; unnamed protein product
Gene Aliases: 7a33; hp51CN; INPP5D; p150Ship; s-SHIP; SHIP; SHIP-1; SHIP1; SIP-145
UniProt ID: (Human) Q92835, (Mouse) Q9ES52
Entrez Gene ID: (Human) 3635, (Mouse) 16331
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
inositol-polyphosphate 5-phosphatase activity
protein binding
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
hydrolase activity
phosphatase activity
SH3 domain binding
phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity
inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity
inositol-4,5-bisphosphate 5-phosphatase activity
phosphatidylinositol trisphosphate phosphatase activity
PTB domain binding
immune system process
apoptotic process
negative regulation of cell proliferation
determination of adult lifespan
negative regulation of signal transduction
immunoglobulin mediated immune response
dephosphorylation
negative regulation of granulocyte differentiation
negative regulation of B cell proliferation
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of interleukin-6 biosynthetic process
positive regulation of B cell differentiation
positive regulation of lymphocyte differentiation
positive regulation of erythrocyte differentiation
negative regulation of monocyte differentiation
negative regulation of neutrophil differentiation
negative regulation of osteoclast differentiation
negative regulation of bone resorption
phosphatidylinositol dephosphorylation
negative regulation of immune response
negative regulation of B cell activation
lipid metabolic process
phosphatidylinositol biosynthetic process
phosphate-containing compound metabolic process
signal transduction
negative regulation of interleukin-6 production
natural killer cell mediated cytotoxicity
negative regulation of natural killer cell mediated cytotoxicity
regulation of immune response
T cell receptor signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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